1. Intro to Labs 1-4
Labs ‘n stuff

2. Lab #1 – Diffusion & Osmosis
4 Parts
1. Diffusion – Qualitative
2. Osmosis – Quantitative & Graph
3. Molar Concentration – Quantitative, Graph, and Inference
4. Water Potential – Calculation
4b. Plasmolysis – if you have not scared or scarred me.

3. Lab #1 – Diffusion & Osmosis
Should get good results
Simple
Understand what you are doing, don’t just follow the steps!!
Think of this lab as intro to AP Labs

4. Lab #1 – Part 1
You will put two substances in a bag made of dialysis tubing.
You will determine whether they exit/enter the dialysis tubing bag.
To do this, you will use either indicator strips or IKI (potassium Iodide or Lugol’s solution)
Data (color) will be recorded in a table

5. Lab #1 – Part 2
Fill dialysis bags with different concentrations of sucrose solution, place bags in beaker with water.
0.0 M
0.2 M
0.4 M
0.6 M
0.8 M
1.0 M
Weigh each (dialysis bag + sucrose solution) BEFORE and AFTER to calculate Percent Change in Mass
Make line graph

6. Lab#1 – Part 2 Why do we use sucrose solution?
What would change if we used a salt solution?
Which bags will increase in mass?
Which bags will decrease in mass?
What is responsible for the increase/decrease in mass?

7. Lab #1 – Part 3
Pieces of potato (or other vegetable) placed in various sucrose solutions
What sucrose concentrations we will use?
Measure mass of potato before being placed in solutions to calculate Percent Change in Mass
Must be left Overnight.

8. Lab#1 – Part 3 Why do we use sucrose solution?
Which potato samples will increase in mass?
Which potato samples will decrease in mass?
What is responsible for the increase/decrease in mass?

9. Lab #1 – Part 4 Calculate Water Potential
Water potential = Solute potential + Pressure potential
Ψ = Ψs + Ψp
Ψs = -iCRT
i = 1
C = Molar Concentration
R = (liter*bar)/(mole*K)
T = temperature in Kelvin

10. What to do next? Organize yourselves into groups of 3-4
Each group is responsible for getting the following:
12 clear or translucent plastic cups (10oz or more)
Sharpie Permanent Marker
Label Cups
Name of group + Period
2 sets of (0.0M, 0.2M, 0.4M, 0.6M 0.8M 1.0M)

11. When? Friday 12/2 Monday 12/5 Tuesday 12/6
Lab #2 Activity (Toothpickase)
Lab #3 Virtual Lab
Monday 12/5
Start Lab #1 & Pre-Lab Quiz (12/5)
Tuesday 12/6
Finish Lab #1
Start Lab #4
Pre-Lab Quiz – Lab #4
TEST: Labs 1-4 on Monday (12/12)

12. Lab #2 – Enzyme Kinetics We will do a modified version of Lab #2
Toothpickase
Self-Contained Lab Exercise
Separate yourself into groups of 3-4
Need:
Approximately 600 flat toothpicks
Approximately 100 round or reinforced toothpicks
Only need to be harder to break than the flat toothpicks

13. Lab #3 – Mitosis & Crossing Over
Virtual Lab Exercise
Data Group Assignment (1-8) by teacher.
First, identify cells in various stages of Mitosis (including interphase)
Second, calculate map units and draw conclusions

14. Lab #4 - Photosynthesis 2nd Actual Lab 2 Parts Fingers Crossed?
Chromatography (Semi-FAIL)
Part that fails miserably
Fingers Crossed?
IF worse comes to worse, you will just analyze given data

15. Lab #4 – Part 1

16. Lab #4 – Calculate Rf values

17. Chromatography
ench/lab4/quiz1.html
Use Online Animations:
ench/lab4/concepts2.html

18. Part that goes well

19. Part that does not go well

20. Results Analysis