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Cellular Respiration Harvesting Chemical Energy
ATP
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ATP The Point is to Make ATP! What’s the point? Whoa! HOT stuff!
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Harvesting stored energy
Energy is stored in organic molecules ____________________________________ ____________ eat these organic molecules food digest organic molecules to get… controlled release of energy “burning” fuels in a series of step-by-step enzyme-controlled reactions We eat to take in the fuels to make ATP which will then be used to help us build biomolecules and grow and move and… live! heterotrophs = “fed by others” vs. autotrophs = “self-feeders”
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Harvesting energy stored in glucose
Glucose is the model ____________________________________ glucose + oxygen carbon + water + energy dioxide respiration + heat C6H12O6 6O2 6CO2 6H2O ATP + Movement of hydrogen atoms from glucose to water fuel (carbohydrates) COMBUSTION = making a lot of heat energy by burning fuels in one step RESPIRATION = making ATP (& less heat) by burning fuels in many small steps ATP glucose enzymes O2 O2 CO2 + H2O + heat CO2 + H2O + ATP (+ heat)
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How do we harvest energy from fuels?
Digest large molecules into smaller ones break bonds & move electrons from one molecule to another as electrons move they “carry energy” with them that energy is stored in another bond, released as heat, or harvested to make ATP • They are called oxidation reactions because it reflects the fact that in biological systems oxygen, which attracts electrons strongly, is the most common electron acceptor. • Oxidation & reduction reactions always occur together therefore they are referred to as “redox reactions”. • As electrons move from one atom to another they move farther away from the nucleus of the atom and therefore are at a higher potential energy state. The reduced form of a molecule has a higher level of energy than the oxidized form of a molecule. • The ability to store energy in molecules by transferring electrons to them is called reducing power, and is a basic property of living systems. loses e- gains e- oxidized reduced + – + e- e- e- REDOX
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How do we move electrons in biology?
Moving electrons in living systems electrons cannot move alone in cells electrons move as part of H atom _____________________________________ p e + H – loses e- gains e- oxidized reduced oxidation reduction Energy is transferred from one molecule to another via redox reactions. C6H12O6 has been oxidized fully == each of the carbons (C) has been cleaved off and all of the hyrogens (H) have been stripped off & transferred to oxygen (O) — the most electronegative atom in livng systems. This converts O2 into H2O as it is reduced. The reduced form of a molecule has a higher energy state than the oxidized form. The ability of organisms to store energy in molecules by transferring electrons to them is referred to as reducing power. The reduced form of a molecule in a biological system is the molecule which has gained a H atom, hence NAD+ NADH once reduced. soon we will meet the electron carriers NAD & FADH = when they are reduced they now have energy stored in them that can be used to do work. C6H12O6 6O2 6CO2 6H2O ATP + oxidation H reduction
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Coupling oxidation & reduction
REDOX reactions in respiration release energy as break down organic molecules break C-C bonds strip off electrons from C-H bonds by removing H atoms C6H12O6 CO2 = __________________________ electrons attracted to more electronegative atoms in biology, the most electronegative atom? O2 H2O = _______________________________ ______________________________________________________________________________________ O2 O2 is 2 oxygen atoms both looking for electrons LIGHT FIRE ==> oxidation RELEASING ENERGY But too fast for a biological system C6H12O6 6O2 6CO2 6H2O ATP + oxidation reduction
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Oxidation & reduction Oxidation Reduction ______________
C6H12O6 6O2 6CO2 6H2O ATP + oxidation reduction
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Moving electrons in respiration
like $$ in the bank Moving electrons in respiration __________________ move electrons by shuttling H atoms around NAD+ NADH (reduced) FAD+2 FADH2 (reduced) reducing power! NAD+ nicotinamide Vitamin B3 niacin P O– O –O C NH2 N+ H NADH P O– O –O C NH2 N+ H adenine ribose sugar phosphates + H reduction Nicotinamide adenine dinucleotide (NAD) — and its relative nicotinamide adenine dinucleotide phosphate (NADP) which you will meet in photosynthesis — are two of the most important coenzymes in the cell. In cells, most oxidations are accomplished by the removal of hydrogen atoms. Both of these coenzymes play crucial roles in this. Nicotinamide is also known as Vitamin B3 is believed to cause improvements in energy production due to its role as a precursor of NAD (nicotinamide adenosine dinucleotide), an important molecule involved in energy metabolism. Increasing nicotinamide concentrations increase the available NAD molecules that can take part in energy metabolism, thus increasing the amount of energy available in the cell. Vitamin B3 can be found in various meats, peanuts, and sunflower seeds. Nicotinamide is the biologically active form of niacin (also known as nicotinic acid). FAD is built from riboflavin — also known as Vitamin B2. Riboflavin is a water-soluble vitamin that is found naturally in organ meats (liver, kidney, and heart) and certain plants such as almonds, mushrooms, whole grain, soybeans, and green leafy vegetables. FAD is a coenzyme critical for the metabolism of carbohydrates, fats, and proteins into energy. oxidation carries electrons as a reduced molecule
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Overview of cellular respiration
4 metabolic stages _______________________ 1. ______________________ respiration without O2 in cytosol respiration using O2 in mitochondria 2. ______________________ 3. ______________________ 4. ______________________ C6H12O6 6O2 6CO2 6H2O ATP + (+ heat)
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What’s the point? ATP The Point is to Make ATP!
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And how do we do that? ___________________ __________________
conformational changes bond Pi to ADP to make ATP allow the H+ to flow down concentration gradient through ATP synthase ADP + Pi ATP ADP + Pi ATP H+
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Got the Energy? Ask Questions!
ADP + Pi ATP H+
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