1. Industriell bruk og nytte av enzymer by Kjartan Sandnes, Dr. Daglig leder og forskningsleder

2. Biorefining salmon co-products Sediment Water soluble protein Oil Water and enzymes Exact time and temp Enzymatic hydrolysis

3. Some key figures Norwegian sea areas 7 times the size of land area All included, Norway is the 13th largest country in the world Second largest seafood export nation 53 bill. NOK 33 mill seafood meals on the table every day

4. White fish (cod, haddock, saithe)325.000 Herring239.000 Aquaculture (salmon, rainbow trout)350.000 Total co-products914.000 «Industrial fish» (to the fish meal- and oil industry 2012)202.000 T O T A L (not for direct human consumption) 1.116.000 Norway 2012: «Non-edible fish raw materials» NOTE! Seaweeds and shellfish not included Source: fhf, Sildesalgslaget

5. Source: fhf, Sintef, Sildesalgslaget

6. Biomega AS – a blue biorefinery 2000:Marine Bioproducts AS established 2002:Production established with patented continuous enzymatic hydrolysis process. No chemical processing aids required 2007: New site acquired for new factory 2011: Six patent families filed so far 2011: 30.000 tons raw materials processed. Turnover 107 mill NOK 2012: New factory with a capacity of 60.000 tons annually (130 mill NOK inv.) - opened May 2012 6

7. Constant efforts to improve quality (cooling systems) and logistics Raw material transportation to Biomega – heads and backbones

8. Page 8 Raw material transportation to Biomega – viscera in bulk by truck

9. 9 New processing plant production from May 2012

10. Overview of new plant 10

11. Biorefining - current and next generation products MB Process Hydrolysate (soluble protein) Oil (fat) Sediment ((unsoluble protein) Bones (minerals, protein) BIOACTIVE PEPTIDES MODIFIED LIPIDS POWDERSMARINE MINERALS PEPTONES PHOSPHOSLIPIDS EXTRACTSGLUCOSAMINES FLAVOURSCHONDROITIN BIOACTIVE PEPTIDES N E W A N D I N N O V A T I V E P R O C E S S E S enzyme technology – extraction – separation - isolation E X A M P L E S

12. Herring Hydrolysates Salmon Hydrolysates Test sampleHH 1HH 2 MB HR 1 MB HR 2 MB HR 3 Peptide size distribution (% of total detected) > 30 000 daltons002.50.20.0 30 000 – 20 000 daltons1.300.70.20.0 20 000 – 10 000 daltons1.20.212.67.14.6 10 000 – 5 000 daltons12.45.217.815.510.0 5 000 - 1 000 daltons14.61013.39.710.2 1 000 - 100 daltons53.166.138.340.533.9 < 100 daltons17.418.414.726.741.3 Rough estimated average Mw21001000490028001900 How enzymes can be used

14. SEM Typical growth medium Nitrogen source Ammonium salts, Nitrates, Peptone (amino acids/peptides) Carbon source Glucose, Glycerol Micronutrients Phosphates, Sulphates, Trace metals, Vitamins BacteriaFungiMammalian cellsYeasts EnzymesAntibioticsVaccinesOther

15. SEM Mask writer highest AN from supplier 1 highest AN from supplier 2 “good for yeasts” from supplier 2

16. Thank you!