1. Control St Control St Control St 3 dai 7 dai 11 dai Protein extraction Tryptic digestion and TMT six-plex labeling Phosphopeptides enrichment by TiO 2 Flowthrough (Total proteome) Eluate (Phosphoproteome) Biological replicate 1 Biological replicate 2 Biological replicate 3 HILIC One run Two runs One run Two runs Phosphopeptides and protein identification and validation Bioinformatics analysis One run 126 127 128 129 130 131 1 2 3 4 5 6 Three biological replicates LC- MS/MS Fig. S1

2. Stakado Sevin A B 3 dai 7 dai 11 dai Stakado Sevin Fig. S2

3. Fig. S3

4. * * * * * * * * * * * * * * Fig. S4