1. Last Time 3/4/08

4. Increase (I) concentration increase slope intercept remains unchanged.

6. Uncompetitive Inhibition Inhibition only has affinity for enzyme-substrate complex Developing the rate law (1) (2)

7. Adding (1) and (2) From (2)

8. Total enzyme

9. Slope remains the same but intercept changes as inhibitor concentration is increased

11. Lecture16 Thursday 3/6/08 Enzyme Kinetics Noncompetive Inhibition Bioreactors Monod Equation Yield Coeficients Washout

14. Bioreactors

19. D=v o / V

26. Noncompetitive, Bioreactors More uncompetitive inhibition Only intercept changes Increasing I No I Lineweaver-Burk Noncompetitive inhibition (Mixed) Both slope and intercept change Increasing I No I E + S E·S P E·I + S E·S·I +I -I

27. Bioreactors Cells + substrate → product + more cells Cell growth can be modeled with enzyme equations since all cell reactors are enzyme catalyzed Lag phase Exponential growth phase stationary phase Death phase Algorithm 1)Mass balance (later) 2)Rates sometimes Product concentration at which all metabolism ceases (93 g/L for wine production, n = ½) Substrate is used to form both products and cell maintenance (rate of substrate disappearance for maintenance

28. 3) Stoichiometry – yield coefficients 1)Mass balance Parameters: 2) Rates