1. Case Study: The world wide distribution of lactose intolerance University of Kentucky: Andrew Bouwma Peter Mirabito Jeffrey Osborn Stanford University: Shyamala Malladi Patricia Seawell University of Miami: Douglas L. Crawford Marjorie F. Oleksiak Facilitators: Kimberly Tanner, San Francisco State Univ. Langdon Martin, Univ Wisconsin

2. Classroom Setting Sophomore Genetics Course Students have been introduced to Genes Molecular Mechanisms of Transcription Transcriptional Regulation Molecular Mechanisms of Translation Translational Regulation

3. Learning Goals –Measurable Outcome Students will understandStudents will be able to (1) the concept of a gene - Sketch a representation of a gene and label its parts (promoter, intron, exon, termination signal) - Relate Mendel’s inheritable traits to a gene (2) the mechanism of transcription - List, order and define the active players in transcription (or diagram) - contrast the structure of nuclear DNA and hnRNA with cytoplasmic mRNA (3) the regulation of transcription - how does exercise lead to larger muscle mass - predict which genes might be expressed under conditions of exercise or hypoxia (low blood oxygen) - how does eating more sugar lead to more absorption of sugars (4) the mechanism of translation - Generate a strip sequence that describes the process/mechanism of translation - Describe how some antibiotics kill bacteria (5) the regulation of translation - compare and contrast global vs mRNA specific regulation - imagine five ways that protein synthesis can be regulated (6) how genes affect phenotypes - explain how skin becomes darker when exposed to the sun - describe how DNA affects the production of hemoglobin - - explain why a person of northern European descent can drink milk, while a majority of the world population cannot (7) and engage in specific components of the scientific process. - read and interpret biogeographical maps - read and apply appropriate information from the primary literature to a scientific problem or question.

4. Learning Goal 6) Genotype  Phenotype 7) Engagement in the Scientific Process Outcomes: Interpret the biogeographical maps Read and apply appropriate information from the primary literature to address a scientific problem. Students will explain why specific populations maintain the ability to drink and digest milk through adulthood.

5. What do you think is the percentage of adults around the world who are lactose intolerant? A:0-20% B: 20-40% C: 40-60% D: 60-80% E:>80%

6. Lactose is milk sugar, a disaccharide in all mammalian milk Lactose intolerance is the inability to digest lactose, because of a lack of the required enzyme lactase in the digestive system.  Drinking milk without having an active lactase enzyme produces gastro-intestinal distress (e.g.,diarrhea).  It is estimated that 75% of adults worldwide show lactose intolerance. Lactose and Lactose Intolerance

7. Adult Worldwide Milk Intolerance due to the Inability to Digest Lactose Milk Intolerance Adapted from http://en.wikipedia.org/wiki/Lactose_intolerance Native Americans Aboriginal Australians

8. Adult Worldwide Lactose Intolerance What do these data say about the worldwide distribution of the ability to drink and digest milk as an adult? Think & Write 1 minute Pair-Share 2 minutes Milk Intolerance

9. Predict What would you predict is the relationship between the world wide distribution of adult and infant lactose intolerance? A: Same B: Opposite C: Unrelated D: Same except for North America Milk Intolerance

10. Discuss Convince your neighbor and revote What is the relationship between the world wide distribution of adult and infant lactose intolerance? A: Same B: Opposite C: Unrelated D: same except for North America

11. What is the relationship between the world wide distribution of adult and infant lactose intolerance? Frequency of Lactose Intolerance in Infants >90% Tolerance Milk Intolerance

12. Brainstorm Given these data: 75% of adults worldwide cannot drink and digest milk Yet nearly all infants are able to digest milk Propose molecular mechanisms to explain why only a few populations have the ability to drink and digest milk throughout adulthood.

13. Choose a hypothesis The molecular mechanism that explains why only a few populations have the ability to drink and digest milk throughout adulthood includes variation in: A: the coding region of the lactase gene B: the regulatory region of the lactase gene C: transcription factors D: the post-translational modification of the enzyme lactase E: None of the above

14. For your final assessment Read the Genetics paper on “Lactose Intolerance” Write a short essay (250 words) to defend one hypothesis and refute one other > Paper will be posted on Blackboard. > Potential hypotheses will be posted. > Biogeographic map will be posted.

15. Diversity: Not using red-green map colors Population diversity Teaching methods addresses a diversity of learning styles Possible discussion of “Got Milk” Activity/AssessmentTopicTime ClickerThink about people you know who are lactose intolerant 1 minute Map InterpretationAnalysis of biogeographical data2 minutes Think and Write Pair-Shore How much diversity is there in lactose intolerance 3 minutes ClickerPredict infant milk intolerance1 minute ClickerDiscuss and revote2 minutes Brainstorm and writePropose molecular mechanisms to explain lactose intolerance 1 minute Clicker pre-assessmentVote on molecular mechanisms to explain lactose intolerance 1 minute Final assessmentDefend and refute hypothesesHomework