1. Dave Marancik, Elizabeth H. Howerth, Roy Burns, Zoli Gyimesi University of Georgia Dept. of Pathology Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

2. Cuban Tree Frog  4 year old, female  Presented formalin-fixed  Part of a zoological collection  Euthanized after a period of anorexia and considerable weight loss  Gross exam: Emaciated Emaciated Diffuse, bilateral corneal opacity Diffuse, bilateral corneal opacity Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

3. Histopathology Diffuse expansion of the cornea Acicular clefts Increased cellularity Scattered plasma cells, lymphocytes, and macrophages Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

4. Corneal lipidosis  Documented in dogs, rabbits, swine, multiple amphibian species Loss of vision frogs and rabbits Loss of vision frogs and rabbits  Causes: Trauma Trauma Dietary Dietary ○ Experimentally reproduced Elevated serum total cholesterol and low-density lipoproteins Elevated serum total cholesterol and low-density lipoproteins Female frogs Female frogs -No evidence linking to vitellogenesis Captive frogs on “normal diet” also developed disease Captive frogs on “normal diet” also developed disease Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

5. Disseminated xanthomatosis Optic nerve Multiorgan diseaseMultiorgan disease CNS and peripheral nerves, lung, gastric submucosa, atherosclerosisCNS and peripheral nerves, lung, gastric submucosa, atherosclerosis Via corneal nerves?Via corneal nerves? Humans have an acquired and familial etiologyHumans have an acquired and familial etiology Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

6. Morphologic diagnosis  Severe, diffuse corneal and peripheral nerve xanthomatosis Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

7. Disseminated xanthomatosis Peripheral nerves Heart Lung Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

8. Welcome to Georgia! Home of … college’s most spirited mascot world’s largest peach cobbler country’s finest athletes Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only