1. AIM: To learn microscope use and parts B3 Agenda: Go over homework Set up lab notebook Pre-lab Microscope lab Warm Up: #1-2 on your homework from last night. Any questions for the others?

2. AIM: To learn microscope use and parts H,B4 Agenda: Go over homework Set up lab notebook Pre-lab Microscope lab Warm Up: Complete the problem on the next slide. Any questions on the homework?

3. Warm Up: Find the IV, DV, constants and control. A scientist wants to see if the soil will yield more potatoes if the soil is fertilized more often. He chooses three identical plots of land and plants the same amount of potato tubers in each. Plot 1 does not get fertilizer, Plot 2 gets 1lb of fertilizer and Plot 3 gets 2lbs of fertilizer. After 100 days, Plot 1 grew 75 potatoes, Plot 2 grew 125 potatoes and Plot 3 grew 150 potatoes. IV: Amount of fertilizer DV: Amount of potatoes yielded Constants: Plot size/soil composition etc. Control: Plot without fertilizer

4. Homework  You need to print for tomorrow:  1. Bio Lab Setup (one-sided)  Tape Pg 1 onto inside of front cover of lab notebook  Tape Pg 2 (safety rules) onto inside of back cover  Tape Pg 5 (grading rubric) onto last page in notebook  2. Print the “What Is Life?” article  3. **Textbook covered & parent form signed

5. Lab Notebook Setup Reference the Biology Lab Notebook Setup on my website. We’ll still go over it in class. You need to print for tomorrow: Bio Lab Setup (one-sided) Tape Pg 1 onto inside of front cover of lab notebook Tape Pg 2 (safety rules) onto inside of back cover Tape Pg 5 (grading rubric) onto last page in notebook

6.  Light Microscope (aka compound light microscope)  Utilize light and glass lenses  Electron Microscopes  Utilize electron beams  EXPENSIVE!  Excellent resolution at HIGH magnifications  Specimen must be non-living for both SEM & TEM  Two types: SEM & TEM

7. Types of Electron Microscopes  Scanning Electron microscope (SEM)  Surface 3D images  Electrons absorbed or pass through-create light and dark spots  Transmission Electron microscope (TEM)  Details of internal cell structure  VERY thin specimens-electrons pass through

8. Pros  Portable  Affordable  Live specimen  Color images Cons  Blurry and dark under high power (resolution not as good)  Specimen must be thin  Light must pass through it  Limits samples possible

9. Pros  Much higher resolution and magnification  Can observe incredibly tiny details Cons  Cannot have living specimens (have to be in a vacuum)  Expensive  More technology required to use it

12. False Color Images- Electron Scopes Pollen GrainsE.coli in intestines

13. Light Microscope SEM

14. TEMSEM

16. In your lab book, create Pg 1 after the table of contents. Your name, lab partner’s name 1 Microscope Lab Purpose: The purpose of this lab is to learn the parts of a microscope, as well as how to use one. Materials:  Microscope  Slides  Cover slips  Salt, sand, sugar, prepared slides, newspaper, cloth, magazine

17. Eyepiece Magnification Objective Magnification Total Magnification Part 1: Part 2: Microscope Parts A. B. C. And so on until J.

18.  Part 3: *** Draw in all 6 circles for Part 3. While you are observing slides, complete questions for Part 4. Some Q’s you need to experiment with the scope to answer them. 1. I’m looking at:2. I’m looking at: Total Magnification:

19. Parts 3 & 4:  You need to view and draw a minimum of:  2 prepared slides  2 depression slides (salt, sugar, sand)  DO NOT USE WATER  2 wet mount slides (cloth, newspaper, magazine)  Create a wet mount  Move on to answer all parts of PART 4

20. Homework  You need to print for tomorrow:  1. Bio Lab Setup (one-sided)  Tape Pg 1 onto inside of front cover of lab notebook  Tape Pg 2 (safety rules) onto inside of back cover  Tape Pg 5 (grading rubric) onto last page in notebook  2. Print the “What Is Life?” article

21. Conclusion  Answer this question in your notebook:  How can we use/have we used microscopy to benefit our society?

22.  Medicine  Discover more about bacteria, parasites, viruses, etc.  Structure almost always determines function- we can learn a lot by discovering what the pathogen looks like  Helps to identify abnormal tissues (like cancer)  Ecology  Observing unique microscopic organisms  Forensic Science  Analyze evidence  Dentistry  Able to see nerves and minute details within the teeth  More accurate procedures