1. Development of LED Lids for Tissue Culture Lighting
Wei FANG*, National Taiwan Univ.
Chi-Chung CHEN, Nano Bio light Technology Co.
Yung-Yi LEE, National Museum of Natural Science
Ming-Yih CHANG, National Ilan University
Nov , 2009
Symposium on Light in Horticulture, Tsukuba, Japan

2. LED
Light up the
Eco-friendly
Dream in Horticulture

3. Distance from lamp to plant is long.
Uniformity on cultural bed is poor.
Space utilization is low (maybe not a problem for research oriented lab.)

4. Usable life span of TFL lamp is short.
Ability to keep consistent output is poor.
Cost to remove extra heat is high.
Relative quantum yield is low, due to too much green light given by TFL.

5. Relative quantum yield

6. Purposes of the study
To develop a TC vessel with LED light source attached.
To develop a TC cart for photo-phyto related research using light source developed.
To develop a TC system for mass production of TC plantlets using carts developed.

7. Absorption spectrums of chlorophyll and phytochrome
Wavelength of LEDs selected
Absorption spectrum
660+5nm
Chl.a 430, 662 nm
Chl.b 453, 642 nm
Phytoch.R 660 nm
Phytoch.FR 730 nm
Absorption spectrums of chlorophyll and phytochrome
450+3nm
525+3nm
730+5nm

8. Results and Discussion
In 2008, development of
TC vessel with double lids
6 types of outer lids with different spectrums (essential 6)
3 stages light intensity control
In 2009 Q1,2
6 spectrums with IR (essential 6+2)

9. Spectral contribution of Essential 6 + 2
401~500nm G
501~600nm R
601~700nm IR
701~800nm
CW (5500 K)
26 % 46 26 2
WW (2700 K) 10 45 41 4
8R1B 13 87
7R1G1B 7 80
3R3B3IR 42 12 6R
100 6B
6IR 14

10. Noted that
8R1B: ratio of number of LED chips used for the particular color
R: 601~700 nm, G: 501~600 nm
B: 401~500 nm, IR: 701~800 nm
8R1B: quantum ratio : 13
7R1G1B: : 7 : 13
3R3B3IR: : : 12

11. A TC vessel with double lids was developed.
outer lid
Inner lid
Inner lid
can be autoclaved
transparent Poly-Carbonate

12. Upper lid of the vessel contains 6 LED lamps, each with 6 chips

13. Distance from light source to plant was greatly reduced.
Elec. Consumption can be reduced.
This research
Others
Distance from light source to plant was greatly reduced.

14. Light distribution highly uniform inside vessel
This research
Others

15. Double the number of layers compare with traditional bench
High space utilization vertically
Double the number of layers compare with traditional bench

16. No pollution from light next to each other.

17. Side dividers prevent pollution from others

18. Light quality adjustable
Blue
9R1B CW
Red WW
8RGB
Change the Lid, change the light quality

19. 8 x 5 ＝40 vessels/layer x 8 layers ＝ 320 vessels/cart
Separate intensity control of each layer
8 x 5 ＝40 vessels/layer x 8 layers ＝ 320 vessels/cart

20. Timer controlled D/N cycle

21. Easy to use
Plug and play

22. Each vessel has separate power input

23. Allowing 5 replicates x 64 treatments
Great tool for Experiment 8 5
Allowing 5 replicates x 64 treatments 8

24. Highly efficient for mass production
Traditional lab.
E-Light
Density
138 vessels/m / (4.5 x 8)
482 vessels/m2
320 / (1.07 x 0.62)
Productivity ratio 1
3.5
Power consumption
2560W (80×32)
1100W
Power consumption ratio
2.5

25. Results and Discussion (cont.)
In 2009 Q1,2 (cont.)
with 10 stages intensity control (0~60 micro-mol/m2/s)
Fine tune on light intensities of different lids

26. 3 stages intensity control
Giant E-Light v1 (2008 HortiFair)
3 stages intensity control

27. 10 stages intensity control
Giant E Light v2a (2009Q1)
10 stages intensity control
六種光蓋在十段光量控制下之比較
Intensities of 10 stages of LED Lids showing 6 types of LED lids (Essential 6)

28. Previous research (unpublished) E-Light prototype system was used
Potato seedlings were grown under different light quantity and quality
Lids
PAR
All R 84
All B 86 CW 66 WW 75 R B CW WW

29. 6 micro mole/m2/s increment per stage
Giant E Light v2b (2009Q3)
6 micro mole/m2/s increment per stage 1 2 3 4 5 6 7 8 9 F
Fine tune on light intensities of different lids

30. Results and Discussion (cont.)
In 2009 Q3
20 spectrums, ‘Excellent 20’
Design of a TC system for mass production using Giant E Light

31. Excellent 20

32. Mass production using 40 ft
Mass production using 40 ft. Cargo container 38 carts/cargo, vessels/cargo
462 vessels/m2

33. Results and Discussion (cont.)
In 2009 Q3 (cont.)
Development of Mini E Light

34. Mini E-Light 2 layers 10 stages intensity control
12 vessels per layer
24 vessels per cart
10 stages intensity control
2 timers for D/N cycle control

35. L107-W62-H195 cm
L66-W40-H80 cm
40 /layer x 8 layers
12 /layer x 2 layers
110VAC/12A
220VAC/5.5A
110VAC/0.61A
220VAC/0.3A

36. Examples of using E-Light for photo-phyto related research
20 types of light quality
10 Intensity levels
Timer(s) for D/N cycle

37. Examples of using E-Light for photo-phyto related research
Group of Lids for research

38. Group of Lids for research: 3R3B in common
3R5B1IR
+3B
+2B1IR
3R3B3IR
3R4B2IR
+3IR
+1B2IR

39. Group of Lids for research: 1R7B in common
1R7B1IR
+1IR

40. Study on the effects of R/B mix light
Lid No. R B
[16] 9
[01] 8 1
[04] 6 3
[08]
[12] 2 7
[14]
[17]

41. Study on the effects of mono-wavelength vs. white LED light
Lid No. R B IR
[16] 9
[17]
[18]
[19]
CW (5500 K)
[20]
WW (2700 K)

42. Study on the effects of R/B mix light with concurrent infrared (1IR or 2IR)
Lid No, R B IR
[03] 7 1
[05] 6 2
[06] 5 3
[09]
[13]
[15]
Lid No.
[07] 4
[10]

43. Study on the effects of supplemental B, R or R/IR mix light with concurrent blue light (3B in common)
Lid No. R B IR
[17]
3 + 6
[04] 6 3
[06] 5 1
[07] 4 2
[11]

44. Study on the effects of supplemental red, infrared or green light with concurrent red and blue light (7R1B in common)
Lid No. R B IR G
[01]
7+1 1
[03] 7
[02]

45. Study on the effects of supplemental red, blue or blue/IR mix light with concurrent red light (3R in common)
Lid No. R B IR
[08] 3 6
[09] 5 1
[10] 4 2
[11]
[16]
3 + 6

46. Study on the effects of B and IR with concurrent R/B mix light
Lid No. R B IR
[04] 6
2+1
[05] 2 1
[12]
6+1
[13]
[14]
7+1
[15] 7

47. Results and Discussion (cont.)
In 2009 Q4
Development of Home growth Light and Control E Light (From LED Lid to LED Panel)

48. Mini E-Light
0~60 umol/m2/s
Home growth Light
0~180 umol/m2/s

49. LED panel made from LED Lids
Home-Growth-Light
(0~180 umol/m2/s)

50. Home Growth-Light Hardening of TC papaya plantlets
Florescent Lamp
LED (8R1B)

51. With Air conditioner and air curtain
Controlled E-Light
With Air conditioner and air curtain

52. LED panel made from LED Lids

53. Conclusions
A TC vessel with double lid was developed. Outer lid equipped with LED lamps. Totally, 20 types of lids are available. Groups of lids for specific research are suggested.

54. Conclusions (cont.) Totally, 4 devices were developed:
Giant E-Light (using LED lid)
Mini E-Light (using LED lid)
Home Growth Light (using LED panel)
Controlled E-Light (using LED panel)

55. Conclusions
A system using giant E-Light in cargo container for mass production of TC plantlets was proposed.

56. Conclusions (cont.)
Posters 18P27, 18P28

57. 2008 Horti Fair

58. 2008 Horti Fair 51

59. 2009 Horti Fair