1. SINKRONISATION SPECIFIK INSTRUCTIONAL PURPOSE STUDENT CAPABLE TO SYNCRONISATION ON LIVESTOCK

2. INTRODUCTION Tecnology Function in Farm : Efficiency of Reproduction Conception Rate Calving Interval Puberty Age Service per Conception

3. Estrous Snap  Make shorter of estrous cycle on a cow Estrous Syncronisation  Make shorter of estrous cycle on a group of livestock

5. ESTROUS SYNCRONISATION Make a group livestock estrous together  Artificial Insemination  gestation  partus of calv with the same age Make a group livestock estrous together  Artificial Insemination  gestation  partus of calv with the same age Efficienci : - cycle shorter - cost of insemination - easy maintenance For teraphy of disease

6. Analisys SWOT of technology estrous sincronisation 1.STRONG 2.WEAKNESS 3.OPPORTUNITY 4.THREAT

7. STRONG -IS CHEAP TECHNOLOGY -NEED COMPETENT PERSONAL -NEW OPPORTUNITY -EFEKTIF FOR ↑ OF REPRODUCTION EFFICIENCY WEAKNESS -PRICE OF HORMONE IS EXPENSIVE -LOW OF GESTATION -LOW INFORMATION -HIGHT COST OF OPERATIONAL FOR LITTLE POPULATION

8. OPPORTUNITY -PERFECTION FOR TECHNOLOGY -NUMBER OF POPULATION ↑ -RERPODUCTION DISTURBANCE ↑ THREAT -AI COST  EXPENSIVE  CHANGE NATURAL MATTING -TRADITIONAL BEHAVIOUR

9. HORMONE APLICATION PROGESTERONE PROSTAGLANDHINE F 2  PRINCIPLE ESTROUS SNAP WITH HORMONE PREPARAT BASED ON FEED BACK PRINCIPLE FROM REPRODUCTION HORMONE

10. PROGESTERONE PRID (PROGESTIN RELEASE INTRAVAGINAL DEVICE) PRID (PROGESTIN RELEASE INTRAVAGINAL DEVICE)  SPIRAL HUMAN  SPIRAL HUMAN CIDR (CONTROL INTRA VAGINAL DEVICE RELEASE) CIDR (CONTROL INTRA VAGINAL DEVICE RELEASE) PRIVASIS (P4 INTRA VAGINAL SILICONS SPONS PRIVASIS (P4 INTRA VAGINAL SILICONS SPONS IMPLANT CYLASTIC => SYNCROMED B  KB SUSUK IMPLANT CYLASTIC => SYNCROMED B  KB SUSUK UNDER SKIN OF NECK / EAR UNDER SKIN OF NECK / EAR PRINCIPLE : AS CL SINTETIC PRINCIPLE : AS CL SINTETIC IMPLANT IN VAGINA 9-14 DAYS IMPLANT IN VAGINA 9-14 DAYS MIN DOSE 40 mg – 1 g MIN DOSE 40 mg – 1 g AS DEPOPROVERA => CONTENT P4 AS DEPOPROVERA => CONTENT P4 THE SURLUS : DIDN’T SEE ESTROUS PHASE THE SURLUS : DIDN’T SEE ESTROUS PHASE

11. WEAKNESS : DIDN’T COMFORTABLE DIDN’T COMFORTABLE IF CONTRACTION => IT’S GO OUT IF CONTRACTION => IT’S GO OUT BETTER : IMPLANTATION UNDER SKIN OF EAR BETTER : IMPLANTATION UNDER SKIN OF EAR IMPLANT IN THE NECK  MAYBE DISAPPEAR IMPLANT IN THE NECK  MAYBE DISAPPEAR IM ???  HOW STOPPED?? IM ???  HOW STOPPED?? SPECIALLY IN SOW  BY FOOD DURING 9 DAYS SPECIALLY IN SOW  BY FOOD DURING 9 DAYS GOOD ESTROUS  P4 COMBINE WITH ESTRADIOL GOOD ESTROUS  P4 COMBINE WITH ESTRADIOL WHY ??? WHY ???

12. HOW ABOUT MECHANISME HOW ABOUT MECHANISME PROGESTERON APLICATION PROGESTERON APLICATION PROSTAGLANDIN F2  APLICATION PROSTAGLANDIN F2  APLICATION

13. PROSTAGLANDINE F2  APLICATION IN LUTEAL PHASE APLICATION IN LUTEAL PHASE THE RULES : THERE IS CL & DIDN’T GRAVID THE RULES : THERE IS CL & DIDN’T GRAVID PG = AS LEMAK, LOW BM = 400 Da So Can Used : I.Ut, PG = AS LEMAK, LOW BM = 400 Da So Can Used : I.Ut, IM, I.Vul, I.Ovr, I.Vn (ABORTUS INDUKTION) IM, I.Vul, I.Ovr, I.Vn (ABORTUS INDUKTION) COMMERCE PREPARAT : COMMERCE PREPARAT : GLANDIN F, GLANDIN F, LUTELYSE, LUTELYSE, PROSOLVIN, PROSOLVIN, PROSTAVET PROSTAVET

14. Lutheal Phase Foliculer Phase 16 – 17 days 4-5 days PG WITH 1 X APLICATION  17/21 x 100 ekor = 75% In Order 100% with 2 X APLICATION PG 1 EST PG2 EST 2-3 Hr 9-14 Hr 75% 100% 9 Days – 3 Days = 6 Days  EARLY OF LUTEAL PHASE 11 Days – 3 Days = 8 Days => in LUTEAL PHASE 11 DAYS

15. COW GOAT/ EWE GOAT/ EWE IM 20-25 mg 7,5 mg I.Ut 2,5 mg I. Vul 7,5 mg 2,5 mg I. Ovr 0,2-0,5 mg - DOSIS PROSTAGLANDIN F2 

17. UTERUS TUBA FALLOPII KORPUS LUTEUM OVARIUM VENA UTERINA ARTERI OVARIKA MEMBELIT VENA VENA ARTERI TRANSFER PG DARI ARTERI MERUSAK KL

22. SUPEROVULATION ↑ COUNT OF OVULATION IN 1 NORMAL ESTROUS PERIODE NEED MANIPULATION TO ↑ OVUM SUPEROVULASI INDUCTION USED HORMONE PREPARAT COMBINE FROM GONADOTROPHINE HORMONE

23. INDIVIDUAL RESPONSE INSUPEROVULATION PROGRAME, DEPENDED ON : POTENTION &HORMONE DOSE HORMON YANG DIBERIKAN COMPARISON OF GONADOTROPHINE FRECUENCY OF INJECTION SPECIES ANIMAL; FAMILY; AGE & FOOD

24. HORMONE APLICATION COMBINATION OF HORMONE FSH & LH PMSG & hCG

25. PMSG (PREGNANT MARE SERUM GONADOTROPHINE) * MARE GESTATION = 40 -120 DAYS. WHY?....FOR WHAT?.... WHY?....FOR WHAT?.... * 120 DAYS DIDN’T HAVE PMSG. WHY…? * IN GESTATION NEED P4  UNTIL 40 DAYS ENAUGH FROM CL GRAVIDITATUM ENAUGH FROM CL GRAVIDITATUM

26. IN 40 DAYS  NEED PMSG  FSH THE FOLICLE GROWTH BUT NOT OVULATION  LUTEINISATION  CL ASCESORIS  PRODUCE P4 CL GRAVID + CL ASCESORIS  P4 ↑ > 120 DAYS  FUNCTION OF P4 FROM > 120 DAYS  FUNCTION OF P4 FROM CL ASCESORIS CHANGED FROM CL ASCESORIS CHANGED FROM PLACENTA UNTIL PARTURITION PLACENTA UNTIL PARTURITION

27. hCG (human Chorionic Gonadotrophine) (human Chorionic Gonadotrophine) hCG  LH from : HUMAN URINE IN GRAVID TRIMESTER I FUNCTION IN HUMAN : STIMULATION OF GONAD IN ORDER TO CL GRAVIDITATUM CONSIST TAKE CARE IN SUPEROVULATION : HELP LH ENDOGEN FOR DISOSIASI FOLICLE WALL IN ORDER TO OVULATION

28. WHEN PMSG INJECTED?? WHEN PMSG INJECTED?? IF FSH LOWER IN BLOOD (LUTEAL PHASE) 9 – 14 DAYS OF ESTROUS CYCLEUS BIRAHI 9 – 14 DAYS OF ESTROUS CYCLEUS BIRAHI IF FSH ↑  DIDN’T BENEFIT IN ORDER TO KNOW  NEED ESTROUS SYNCRONISATION ONLY 1 INJECTED

29. LONG ACTION = 108 HOURS (5-6 DAYS) LONG ACTION = 108 HOURS (5-6 DAYS) ESTROGENIK EFECT  DISTURB OF ESTROGENIK EFECT  DISTURB OF IMPLANTATION IMPLANTATION DOSE 2000 – 3000 IU (FOLIGON) DOSE 2000 – 3000 IU (FOLIGON) FOR OVUM PRODUCE  DIDN’T NEED FOR OVUM PRODUCE  DIDN’T NEED MATTING MATTING FOR ET  NEED MATTING FOR ET  NEED MATTING PHYSIOLOGIS CONDITION OF DONOR PHYSIOLOGIS CONDITION OF DONOR MUST THE SAME OF RESIPIEN MUST THE SAME OF RESIPIEN HOW ABOUT PMSG??

30. DONOR RESIPIEN PG1 11Day PG2 9Day 2Day PG3 3Day EST 7Day Flushing PMSG PG1 11 Day PG2 3Day EST 7 Day Transfer hCG IB Pagi IB Sore 1/6 12/6 21/6 23/6 26/6 3/7 SUPEROVULATION with PMSG & hCG – Sinkron with PG SUPEROVULATION with PMSG & hCG – Sinkron with PG DOSE of PMSG : 2000 – 3000 IU DOSE of hCG : 1500 -2000 IU 12/6 21/6 23/6 26/6 3/7

31. DONOR RESIPIEN PRID 9 Day 3 Day EST 7 Day Flushing PMSG PRID 9 Day 3 Day EST 7 Day Transfer hCG IB Pagi IB Sore 1/6 10/6 13/6 20/6 SUPEROVULATION (PMSG-hCG) - Sinkron (P4) SUPEROVULATION (PMSG-hCG) - Sinkron (P4) DOSIS PMSG : 2000 – 3000 IU DOSIS Hcg : 1500 -2000 IU DOSIS P4 DALAM PRID = 40 mg – 1 g 1/6 10/6 13/6 20/6 OUT KOMBINASI PG BIRAHI LEBIH BAGUS OUT

32. WHEN FSH INJECTED?? WHEN FSH INJECTED?? BOOSTER INJECTED (4 DAYS: MORNING & EVENING) (4 DAYS: MORNING & EVENING) EVERY 12 HOURS COUNT 40 mg WITH DOSE DECREASE : 6, 5, 4, 3 mg IN ORDER TO KNOW  NEED ESTROUS SYNCRONISATION MASA KERJA PENDEK S/ TDK BERSIFAT ESTROGENIK S/ PEROLEHAN EMBRIO DG KWALITAS LEBIH BAIK U/ PROD. OVUM TDK PERLU DIKAWINKAN U/ ET PERLU DIKAWINKAN SYARAT KONDISI FISIOLOGIS DONOR=RESIPIEN

33. SHORT ACTION = 12 HOURS SHORT ACTION = 12 HOURS DIDN’T ESTROGENIK EFECT DIDN’T ESTROGENIK EFECT KWALITAS EMBRYO IS BETTER GOODKWALITAS EMBRYO IS BETTER GOOD FOR OVUM PRODUCE  DIDN’T NEEDFOR OVUM PRODUCE  DIDN’T NEED MATTING MATTING FOR ET  NEED MATTING FOR ET  NEED MATTING PHYSIOLOGIS CONDITION OF DONOR PHYSIOLOGIS CONDITION OF DONOR MUST THE SAME OF RESIPIEN MUST THE SAME OF RESIPIEN HOW ABOUT FSH??

34. DONOR RESIPIEN PG1 11Day PG2 9 Day PG3 3Day EST 7Day Flushing FSH PAGI PG1 11 Day PG2 3Day EST 7Day Transfer LH IB Pagi IB Sore 1/6 12/6 21/6 22/6 23/6 24/6 27/6 4/7 SUPEROVULATION WITH FSH & LH SUPEROVULATION WITH FSH & LH FSH I : 6 mg FSH II : 5 mg FSH III : 4 mg FSH IV : 3 MG TOTAL 40 mg FSH PAGI FSH PAGI FSH PAGI 12/6 23/6 27/6 4/7 FSH SORE FSH SORE FSH SORE FSH SORE