1. From Genome to Proteome Juang RH (2004) BCbasics Systems Biology, Integrated Biology

2. Proteome Reflects Gene Expression Repertoire Juang RH (2005) BCbasics Proteolytic digestion Proteolytic fragments Pure protein2D electrophoresis Sample MALDI-TOF Capillary Electrophoresis HPLC N- Amino acid sequencing Mass spectrum Database searching LC/MS/MS

3. MALDI-TOF Protease digestion ● Compare peptides and identify the unknown protein m/z P1P2P3P4 MW4MW2MW3MW1 Unknown protein Search Database Candidate protein Digestion Simulation MW4MW2MW3MW1 Calculate Mol wt Identify Unknown Protein by Mass Juang RH (2004) BCbasics

4. LC ESI-Mass/Mass Protein Sequencing by Mass Protease digestion P1P2P3P4 Unknown protein P4 G K G S W V R -GKGSWVR ● Direct sequencing P1P2P3 ESI-Mass/Mass P4 P LC P4 P ● Phosphorylation site Protease digestion Phosphorylated protein Juang RH (2004) BCbasics