1. Regional Breeding for Wheat Stripe Rust Resistance in the Eastern United States David Marshall Plant Science Research Unit Raleigh, NC

2. The U.S. has the most cost- effective and plentiful food production and supply system in the world.

3. Wheat Stripe Rust

4. Epidemic Year % Loss Stripe Rust Epidemic Years and the States with Greater than 1% Yield Loss from 1976 to 2004.

5. Objectives:  Identify stripe rust resistance/susceptibility in Eastern U.S. wheat cultivars, elite lines, and other germplasm.  Identify the gene or genes involved resistance.  Introgress stripe rust resistance into elite lines having regional/local adaptation.  Distribute populations and fixed lines to cooperators.

6. Protocol:  Established the Uniform Eastern Wheat Stripe Rust Nursery. In 2005-06, had up to 20 entries from State breeding programs in FL, GA, NC, VA, MD, NY, MI, MO, IL, IN, KY, AR, and LA; private breeding programs – Westbred, AgriPro-Coker, and Genesis; and USDA/ARS.  Seedling screening in Raleigh, NC.  Field screening in Plains and Griffin, GA; Kinston, NC; Baton Rouge and Winnsboro, LA; Fayetteville, AR; San Antonio, TX, Pullman and Mt. Vernon, WA.

7. IT% DesignationPedigreeContributorSourceMean TERRAL LA841 GA8665-D4/GA85238-C3HarrisonLouisiana0.00 GA961567- 4A35 Jackson/2*881130JohnsonGeorgia0.00 GA97531- 5A37 891138/Kinsco//PIO 2628JohnsonGeorgia0.00 LA99042E- 62-B Ducula1(H2O)/Mason,F1//PIO26R61HarrisonLouisiana0.750.03 LA96140BU A70-2 VA88-52-69/TX89D6435//C9134,F1/3/MasonHarrisonLouisiana0.750.03 GA951231- 4E26 881130/C9134JohnsonGeorgia0.250.03 NC03-7222L910568/NCV93-1007//LA854111MurphyNorth Carolina 0.250.03 MO 050146MO 94-046/Pioneer 2552McKendryMissouri0.250.03 IL87-2834-1Tyler / HowellKolbIllinois0.750.03 992133A2- 1-2 Patterson/F201R//INW0101/4/Patton/3/Clark*4/Ning 7840//Foster OhmIndiana0.250.03

8. Identified 102 lines with resistance better than the "resistant" check (Pioneer 26R61). Infection types less than '3' and severities less than 18%. Identified adult-plant resistance in at least 8 lines: LA95171CA58-3- 2-CCK9134/PIO2684,F1//90239A29-1-X(CL850643/PIO2548//C9877) 97C-0554-01VA94-54-549/Roane//Kristy B010973L880085/XW502 Coker 9511 L881060/L880436 IL87-2834-1Tyler / Howell IL02-5030P88288C1-1-6-1-2-8 / 96-6472 IL03-1843897-3574 / 95-4162 INW0412Huapei 57-2/Patterson

9. Several lines had combined stripe rust, leaf rust and powdery mildew resistance. FL91226A-X4Star'S'(IBW179)/C9105//F302/P2550,F5IDEO FL98031-D15-E48952-B-7/CM98322-0M-2Y,F1//P26R61 LA95171CA58-3-2-CCK9134/PIO2684,F1//90239A29-1-X(CL850643/PIO2548//C9877) LA98094-BUB-58-590185B24-4-3/87167D8-2 GA 96229-3A41881130/*2 881582 GA961567-4A35Jackson/2*881130 GA981621-5E34AGS 2485/PIO 26R61 GA98249G1-5E56881130/ 2*901146 GA011450DH2-5A45USG 3592/PIO 26R61 ARS03-3849TX85-264*2/TTCC512 (T.cylindricum) (=WX02ARS149-8) VA01W-205PION2684/93-54-185 (WLR/3/MSY*3/BALKAN//SAL)// POCAHONTAS,F11 B990133 L890145/LX 8728D INW0412Huapei 57-2/Patterson 99840C4-8-4961331/INW0102//F201R/Foster E01-1053U90-1A//ZX90-2C1/P2580

10. Regional Breeding scheme for introducing resistant sources: A. Backcross-Bulk (minor genes) 1. Several resistant sources x Adapted type 2. Backcross to Adapted type 3. Select in BC1 through F3 for low terminal severities B. Accelerated Population Development for major genes 1. Resistant source x Adapted type (crosses made in February) 2. F1 planted in NZ in May, F2 in NC in Nov, F3 in NZ in June, F4 in in Nov.