1. The Endogenous Cannabinoids and the Control of Drug Craving Alexandros Makriyannis, Director

3.  9 /  8 -THC  9 -THC /  8 -THC  9 -tetrahydrocannabinol  8 -tetrahydrocannabinol

4. Medicinal Uses for Cannabinoids AnalgesicAnticonvulsiveSedativeAntidepressiveHypnoticAntiasthmaticImmunomodulation Memory Enhancing Anesthetic Birth Control Appetite Stimulation Antipyretic Neuropathic Pain AntiemeticAntirheumaticAntimigraineAntineuralgic Reduction of Fatigue

5. Two Cannabinoid Receptors: CB1 & CB2 174 179 CB2 e1 e2 e3 i1 i2 i3 Extracellular 472 amino acids 360 amino acids

7. Endogenous Cannabinoid Receptor Ligands Anandamide2-Arachidonylglycerol Palmitylethanolamide CB2 72279 CB1CB2CB1 K i (nM) 58 145

9. Cannabinoid Targets for Drug Discovery

10. Nature Neuroscience, Vol. 2, 4, 304-5 (1999)

11. Retrograde Signaling & the Cannabinergic System

12. Purification of FAAH MW: 63 kDaMW: 63 kDa pI: 6.2pI: 6.2208144 87 44.1 32.7

13. Inhibition of [ 3 H]anandamide accumulation by AM 404 and AM403 Science, Vol. 277, 1094-97 (1997) AM404 AM403

14. Pharmacological Tools

15. Cannabinergic Ligands O N H OH AM 251 Anandamides (AEA’s) AM356 1,5-Biarylpyrazoles  8 -THC Aminoalkylindoles(AAI’s) Non-Classical (NCC’s) AM630 OH OH O H Classical (CC’s) CP55,940