1. The Spinal Cord The Spinal Cord Basic Neuroscience James H. Baños, Ph.D.

2. Grey and White Matter

3. Grey Matter = Cell Body White Matter = Myelinated axon

4. Grey and White Matter Grey matter Grey matter Cortex Cortex Nucleus (CNS) Nucleus (CNS) Ganglion (PNS) Exception: Basal Ganglia Ganglion (PNS) Exception: Basal Ganglia

5. Grey and White Matter White Matter White Matter Nerve (PNS) Nerve (PNS) Tract (CNS) Tract (CNS) Fasciculus/Funiculus -- Group of fibers with common origin and destination Fasciculus/Funiculus -- Group of fibers with common origin and destination Lemniscus -- Ribbon-like fiber tract Lemniscus -- Ribbon-like fiber tract Peduncle -- Massive group of fibers -- usually several tracts Peduncle -- Massive group of fibers -- usually several tracts

6. Grey and White Matter Tracts are named with origin first, then destination Tracts are named with origin first, then destination Corticospinal tract -- cortex to spinal cord Corticospinal tract -- cortex to spinal cord Mammilothalamic tract -- Mammilary bodies to thalamus Mammilothalamic tract -- Mammilary bodies to thalamus Spinocerebellar tract -- Spinal cord to cerebellum Spinocerebellar tract -- Spinal cord to cerebellum Corticobulbar tract -- Cortex to brain stem Corticobulbar tract -- Cortex to brain stem

7. The Spinal Cord

8. General Organization Spinal cord is SMALL! Spinal cord is SMALL! 42-45 cm long 42-45 cm long 1 CM wide at widest point 1 CM wide at widest point Does not extend all the way to the bottom of the spinal column Does not extend all the way to the bottom of the spinal column Pattern of grey/white matter is reversed in the cord Pattern of grey/white matter is reversed in the cord White matter tracts on outside White matter tracts on outside Grey matter on the inside Grey matter on the inside Staining reverses this!!! Staining reverses this!!!

9. General Organization White matter (tracts of axons) Grey Matter (cell bodies)

10. General Organization Spinal cord is segmented anatomically Spinal cord is segmented anatomically Input and output occurs in groups of rootlets arranged in a series longitudinally along the cord Input and output occurs in groups of rootlets arranged in a series longitudinally along the cord Dorsal rootlets -- Input -- carry sensory information Dorsal rootlets -- Input -- carry sensory information Ventral rootlets -- Output -- motor neurons Ventral rootlets -- Output -- motor neurons

11. General Organization Each set of rootlets forms a spinal nerve that innervates a corresponding segment of the body, called a dermatome Each set of rootlets forms a spinal nerve that innervates a corresponding segment of the body, called a dermatome

12. General Organization

13. There are 31 segments in the spinal cord: There are 31 segments in the spinal cord: 8 cervical (C1 - C8) 8 cervical (C1 - C8) 12 Thoracic (T1 - T12) 12 Thoracic (T1 - T12) 5 Lumbar (L1 - L5) 5 Lumbar (L1 - L5) 5 Sacral (S1 - S5) 5 Sacral (S1 - S5) 1 Coccygeal 1 Coccygeal

14. General Organization The spinal cord is housed within the vertebral column The spinal cord is housed within the vertebral column

15. General Organization Each cord segment has a corresponding vertebra of the same name (e.g., C3) Each cord segment has a corresponding vertebra of the same name (e.g., C3) Spinal nerves enter/exit underneath their corresponding vertebral segment Spinal nerves enter/exit underneath their corresponding vertebral segment

16. General Organization But wait! Something doesn’t add up! But wait! Something doesn’t add up! How can spinal nerves exit below their corresponding vertebral segment if the cord is only 42cm-45cm long? How can spinal nerves exit below their corresponding vertebral segment if the cord is only 42cm-45cm long? Answer: Spinal nerves extend down to the appropriate vertebral segment forming the cauda equina Answer: Spinal nerves extend down to the appropriate vertebral segment forming the cauda equina This means cord segments and vertebral segments don’t line up This means cord segments and vertebral segments don’t line up

17. General Organization

18. Cord is not of uniform thickness throughout its length. Why not? Cord is not of uniform thickness throughout its length. Why not?

19. General Organization Cord is not of uniform thickness throughout its length. Why not? Cord is not of uniform thickness throughout its length. Why not? Answer: Answer: Segments of the cord innervate parts of the body that differ in complexity Segments of the cord innervate parts of the body that differ in complexity There are fewer white matter tracts lower in the cord. There are fewer white matter tracts lower in the cord.

20. General Organization Cervical enlargement C5 - T1 Lumbar enlargement L2 - S3

21. The Spinal Cord in Cross Section

22. Cord Sections Segments of the spinal cord have a similar organization, but vary in appearance. Segments of the spinal cord have a similar organization, but vary in appearance. Always know where you are in the cord (i.e., cervical, thoracic, lumbar, sacral) Always know where you are in the cord (i.e., cervical, thoracic, lumbar, sacral)

23. Cord Sections -- Cervical Cervical cord is wide, flat, almost oval in appearance. Why? Cervical cord is wide, flat, almost oval in appearance. Why?

24. Cord Sections -- Cervical Enlargement Cervical Cervical Enlargement What’s different about the cervical enlargement. Why? What’s different about the cervical enlargement. Why?

25. Cord Section -- Thoracic Less White matter than cervical Less White matter than cervical Rounder appearance Rounder appearance Less prominent ventral horns than cervical enlargement Less prominent ventral horns than cervical enlargement

26. Cord Section -- Lumbar Less White matter than thoracic Less White matter than thoracic Rounder appearance Rounder appearance Larger ventral horns, especially in lumbar enlargement Larger ventral horns, especially in lumbar enlargement Lumbar Lumbar Enlargement

27. Cord Section -- Sacral Not much white matter Not much white matter Mostly grey, although not much of that either Mostly grey, although not much of that either

29. Cross Sectional Organization Anterior median fissure Anterior white commisure Posterior median sulcus Posterior intermediate sulcus Tract of Lissauer

30. Grey Matter Laminar Laminar Laminae of Rexed Laminae of Rexed

31. Grey Matter Posterior (dorsal) Horn Posterior (dorsal) Horn Intermediate Grey Intermediate Grey Anterior (ventral) Horn Anterior (ventral) Horn

32. Grey Matter: Posterior Horn Mostly Interneurons Mostly Interneurons Substantia gelatinosa Substantia gelatinosa Pain/temp proc Pain/temp proc Body of the posterior horn Body of the posterior horn Sensory proc Sensory proc

33. Grey Matter: Intermediate Grey Clarke’s Column Clarke’s Column T1-L3 T1-L3 Balance/proprio. Balance/proprio. Intermediolateral Column Intermediolateral Column T1-L3 T1-L3 Sympathetic neurons Sympathetic neurons

34. Grey Matter: Anterior Horn Lower Motor Neurons Lower Motor Neurons

35. Corticospinal tract Dorsal Columns Spinothalamic tract Spinocerebellar tracts White Matter: The “Big Four” Pathways

36. The Big Four Corticospinal tract Voluntary motor Dorsal columns/ medial lemniscus Discriminative touch Conscious proprioception Spinocerebellar tract (dorsal and ventral) Unconscious proprioception Spinothalamic tract Pain/temperature

37. Corticospinal Tract Voluntary Motor

38. Corticospinal Tract First order neuron (upper motor neuron) originates in precentral gyrus First order neuron (upper motor neuron) originates in precentral gyrus Passes through internal capsule Passes through internal capsule 90% decussates in caudal medulla 90% decussates in caudal medulla Lateral corticospinal tract Lateral corticospinal tract 10% undecussated 10% undecussated Anterior corticospinal tract Anterior corticospinal tract Synapses on second order neuron (lower motor neuron) in ventral gray of the cord Synapses on second order neuron (lower motor neuron) in ventral gray of the cord Second order neuron innervates muscle Second order neuron innervates muscle

39. Motor Homunculus HAL

40. Motor Homonculus Head Arms Legs HAL:

41. Corticospinal Tract Spinal Cord Medulla PonsMidbrain

42. Upper & Lower Motor Neurons Upper Motor Neuron Upper Motor Neuron Motor Cortex to Ventral Grey Horn Motor Cortex to Ventral Grey Horn Modulatory influence on stretch reflex arc Modulatory influence on stretch reflex arc Lower Motor Neuron Lower Motor Neuron Ventral Grey Horn to Neuromuscular Junction Ventral Grey Horn to Neuromuscular Junction Efferent of stretch reflex arc Efferent of stretch reflex arc Helps maintain tone Helps maintain tone Sensory Neuron Sensory Neuron Stretch receptors in muscle and tendons Stretch receptors in muscle and tendons Helps maintain tone Helps maintain tone Afferent of basic stretch reflex arc Afferent of basic stretch reflex arc Motor Ctx Ventral Grey Horn UMN LMN

43. Upper & Lower Motor Neurons Maintenance of Tone Maintenance of Tone Input from stretch receptors causes lower motor neuron to supply tonic stimulation to the muscle Input from stretch receptors causes lower motor neuron to supply tonic stimulation to the muscle The upper motor neuron modulates this -- will tend to “override” the tonic signal from the sensory neuron The upper motor neuron modulates this -- will tend to “override” the tonic signal from the sensory neuron UMN LMN

44. Upper & Lower Motor Neurons Reflex Arc Reflex Arc Afferent is sensory neuron detecting a sudden stretch Afferent is sensory neuron detecting a sudden stretch Signal is strong and results in a strong response by the lower motor neuron Signal is strong and results in a strong response by the lower motor neuron Strong signal usually overcomes mild cortical input from the UMN Strong signal usually overcomes mild cortical input from the UMN UMN LMN

45. Upper & Lower Motor Neurons Upper Motor Neuron Signs Upper Motor Neuron Signs Spastic paresis Spastic paresis Hypertonia Hypertonia Hyperreflexia Hyperreflexia No muscle atrophy (until perhaps late in the course) No muscle atrophy (until perhaps late in the course) Positive Babinski Positive Babinski Why? Why? Loss of voluntary UMN signal Loss of voluntary UMN signal Loss of modulation of tone and reflexes by UMN -- the circuit runs unchecked Loss of modulation of tone and reflexes by UMN -- the circuit runs unchecked Motor Ctx Ventral Grey Horn UMN LMN

46. Upper & Lower Motor Neurons Lower Motor Neuron Signs Lower Motor Neuron Signs Flaccid paresis/paralysis Flaccid paresis/paralysis Muscle fasciculations Muscle fasciculations Hypotonia Hypotonia Hyporeflexia Hyporeflexia Muscle atrophy Muscle atrophy Negative Babinski Negative Babinski Why? Why? Loss of LMN for voluntary movement Loss of LMN for voluntary movement Loss of efferent component of reflex arc and tone pathway Loss of efferent component of reflex arc and tone pathway Motor Ctx Ventral Grey Horn UMN LMN

47. Babinski’s Sign In response to stimulation of the sole of the foot, the toes will usually curl downward. In response to stimulation of the sole of the foot, the toes will usually curl downward. When UMN inhibition is removed, the toes will curl upward (Dorsiflexion). This is referred to as a positive Babinski or presence of Babinski’s sign. When UMN inhibition is removed, the toes will curl upward (Dorsiflexion). This is referred to as a positive Babinski or presence of Babinski’s sign.

48. Related Terms… Spasticity -- Increased muscle tone and increased reflex contraction (UMN) Spasticity -- Increased muscle tone and increased reflex contraction (UMN) Clonus -- Rythmic contractions and relaxations seen when a spastic muscle is stretched (UMN) Clonus -- Rythmic contractions and relaxations seen when a spastic muscle is stretched (UMN)

49. Basics of Localization If all limbs are checked for upper and lower motor neuron signs, you can begin to localize lesions If all limbs are checked for upper and lower motor neuron signs, you can begin to localize lesions Left-right differences are also very important Left-right differences are also very important

50. Dorsal Column/ Medial Lemniscus Discriminative Touch Conscious Proprioception

51. Dorsal Columns/Medial Lemniscus First order neuron begins in receptor First order neuron begins in receptor Enters cord at tract of Lissauer Enters cord at tract of Lissauer Legs run in fasciculus gracilis (medial dorsal) Legs run in fasciculus gracilis (medial dorsal) Arms run in fasciculus cuneatus (lateral dorsal) Arms run in fasciculus cuneatus (lateral dorsal) Synapse on nucleus gracilis and nucleus cuneatus (caudal medulla) Synapse on nucleus gracilis and nucleus cuneatus (caudal medulla) 2nd order neuron decussates and runs from NG & NC to thalamus (as medial lemniscus) 2nd order neuron decussates and runs from NG & NC to thalamus (as medial lemniscus) 3rd order neuron runs from thalamus to postcentral gyrus 3rd order neuron runs from thalamus to postcentral gyrus

52. Dorsal Columns/Medial Lemniscus Spinal Cord Medulla PonsMidbrain

53. Spinocerebellar Tracts Unconscious Proprioception

54. Dorsal (Posterior) Spinocerebellar Tract Involves Clark’s Column, a longitudinal gray matter body from about T1 to L3 Involves Clark’s Column, a longitudinal gray matter body from about T1 to L3 Below Clark’s Column: Below Clark’s Column: Runs with f. cuneatus, synapses in Clark’s Column, joins dorsal spinocerebellar tract Runs with f. cuneatus, synapses in Clark’s Column, joins dorsal spinocerebellar tract Level of Clark’s Column Level of Clark’s Column Synapses in Clark’s Column, joins dorsal spinocerebellar tract Synapses in Clark’s Column, joins dorsal spinocerebellar tract Above Clark’s Column Above Clark’s Column Runs with f. cuneatus, synapses in lateral cuneate nucleus (caudal medulla), projects to ipsilateral cerebellum Runs with f. cuneatus, synapses in lateral cuneate nucleus (caudal medulla), projects to ipsilateral cerebellum

55. Dorsal (Posterior) Spinocerebellar Tract Spinal Cord Medulla PonsMidbrain To Cerebellum L3 T1

56. Ventral (Anterior) Spinocerebellar Tract Supplements Dorsal Spinocerebellar Tract Supplements Dorsal Spinocerebellar Tract Information from more diverse array or receptors Information from more diverse array or receptors Originates from scattered cells in the intermediate grey caudal to L1 (which in turn have input from proprioceptive axons or their collaterals Originates from scattered cells in the intermediate grey caudal to L1 (which in turn have input from proprioceptive axons or their collaterals Crosses twice, to end up in ipsilateral cerebellum Crosses twice, to end up in ipsilateral cerebellum

57. Ventral (Anterior) Spinocerebellar Tract Spinal Cord Medulla PonsMidbrain

58. Spinothalamic Tract Pain and Temperature

59. Spinothalamic Tract First order neurons originate in pain receptors, enter cord at tract of Lissauer, and synapse in substantia gelatinosa or nucleus proprius First order neurons originate in pain receptors, enter cord at tract of Lissauer, and synapse in substantia gelatinosa or nucleus proprius Second order neurons cross at the anterior white commissure, rising 1 or 2 cord levels in the process, and form contralateral spinothalamic tract Second order neurons cross at the anterior white commissure, rising 1 or 2 cord levels in the process, and form contralateral spinothalamic tract A third order neuron (not technically spinothalamic tract) projects to the cortex A third order neuron (not technically spinothalamic tract) projects to the cortex

60. Spinothalamic Tract

61. Spinal Cord Medulla PonsMidbrain

62. L1 L2 L3 L4 L5 L1 L2 L3 L4 L5

63. Coming Up… Lab Week Overview: Monday Lab Week Overview: Monday Virtual Labs Virtual Labs Wet lab day: Thursday Wet lab day: Thursday Lab Practical Exam: Friday Lab Practical Exam: Friday