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1.96E+07 3.9E+07 6.6 E+07 3.4E+07 6.7E+07 5.7E+07 0E+0 1E+7 2E+7 3E+7 4E+7 5E+7 6E07 7E+7 8E+7 9E+7 hWASP1 Cell Growth 234234 Cell Number Days of Growth.

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Presentation on theme: "1.96E+07 3.9E+07 6.6 E+07 3.4E+07 6.7E+07 5.7E+07 0E+0 1E+7 2E+7 3E+7 4E+7 5E+7 6E07 7E+7 8E+7 9E+7 hWASP1 Cell Growth 234234 Cell Number Days of Growth."— Presentation transcript:

1 1.96E+07 3.9E+07 6.6 E+07 3.4E+07 6.7E+07 5.7E+07 0E+0 1E+7 2E+7 3E+7 4E+7 5E+7 6E07 7E+7 8E+7 9E+7 hWASP1 Cell Growth 234234 Cell Number Days of Growth (post seed) = 4 x10 6 seed, 40 ml, T175 = 2 x10 6 seed, 40 ml, T175 = 8 x10 6 seed, 40 ml, T175 Supplemental Figure 1

2 1.3E+07 1.6E+07 1.3E+07 2.3E+07 3.4E+07 0 10 100 1e+3 1e+4 1e+5 1e+6 1e+7 1e+8 12388 Stability of 650MNDhWASp Titers IU/ml Weeks in culture before 4 day induction Supplemental Figure 2

3 0 5 10 15 20 25 30 35 40 45 16.3 9.9 30 8.7 26 2.9 8.2 14.2 23 %Hu CD45 + Cells engrafted in NSG BM 50 a. 0 10 20 30 40 50 60 70 80 90 100 37 33 7.0 48 25 55 26 31 16.7 1hit MOI 100 1hit + DMSO 1hit + DMSO+ Ex527 1hit + DMSO+ CHIR98014 1hit + NAM 2hits MOI 100 1hit MOI 200 1 hit MOI 200 + NAM 1hit MOI 200+ NAM+ SDF1  54 42 0 38 17 = 12 methocult cultures %hW + CFU-Cs from NSG BM 57 42 21 14 b. Supplemental Figure 3 p = 0.0188 p = 0.0198 p = 0.0066 p = 0.116 p = 0.38

4 Mock +DMSO 650+ DMSO 650+ SDF1  +DMSO 650+ 50 nM Rapa for entire culture a. b. Supplemental Figure 4 p = 0.021 p = 0.133 p = 0.0153 %CD45 + Cells % Engrafted %hWASP Vector + %CFU-Cs 26 24 27 48 0 10 20 30 40 50 60 70 0.0 8.1 17.8 1.1 0 10 20 30 40 50 60


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