1. From turned off…..to tuned in (Start the Slide Show. This presentation is automatically timed: 3.75 minutes) Tips to construct and display complex research data Most Postdoctoral researchers see the difference. Those who follow these tips make a difference.

2. Components of the canonical JNK pathway in dorsal closure are required for wound closure ? msn slpr hep bsk response DJunDFos MAP4K MAP3K MAP2K MAPK Txn factors canonical JNK pathway control MAP4KMAP3KMAP2K MAPK Txn factors w 1118 msn x2 slpr Tak1 x2 Takl1 Takl2 Pk92BMekk1 hep x2 Mkk4 bsk x2 DJun DFos 100 80 60 40 20 0 15 open wounds [%] † † wound reporter:e22c-Gal4A58-Gal4Dcr-2;A58-Gal4 † lethal cross N = ≥ 30 Here’s the original slide ^ Oversized headline sucks almost a third of the space? Navigation/legend sucks > half of what remains? < Leaving only 25% of space for the research data!?

3. Components of the canonical JNK pathway in dorsal closure are required for wound closure ? msn slpr hep bsk response DJunDFos MAP4K MAP3K MAP2K MAPK Txn factors canonical JNK pathway control MAP4KMAP3KMAP2K MAPK Txn factors w 1118 msn x2 slpr Tak1 x2 Takl1 Takl2 Pk92BMekk1 hep x2 Mkk4 bsk x2 DJun DFos 100 80 60 40 20 0 15 open wounds [%] † † wound reporter:e22c-Gal4A58-Gal4Dcr-2;A58-Gal4 † lethal cross N = ≥ 30 Over 106 elements scream for attention +7+7 +6+6 +23+23+4+4 11 +11+11 +17+17 +13+13 +10+10 +9+9 +5+5

4. Components of the canonical JNK pathway in dorsal closure are required for wound closure control MAP4KMAP3KMAP2K MAPK Txn factors w 1118 msn x2 slpr Tak1 x2 Takl1 Takl2 Pk92BMekk1 hep x2 Mkk4 bsk x2 DJun DFos 100 80 60 40 20 0 15 open wounds [%] † † ? msn slpr hep bsk response DJunDFos MAP4K MAP3K MAP2K MAPK Txn factors canonical JNK pathway wound reporter:e22c-Gal4A58-Gal4Dcr-2;A58-Gal4 † lethal cross N = ≥ 30 And here’s how it was animated

5. This is what’s called a Treasure Hunt Audiences take in data from top-to-bottom, left-to-right Let’s rebuild this slide, starting with a roadmap

6. ? msn slpr hep bsk DJun DFos response Canonical JNK pathway MAP4K MAP2K MAP3K Txn factors MAPK ? msn slpr hep bsk DJun DFos response Canonical JNK pathway MAP4K MAP2K MAP3K Txn factors MAPK Components of canonical JNK pathway in dorsal closure are required for wound closure First display the title: “what we’ll discuss” Next: “how we plan to get there” (organization of data sets) Now! We’re ready to share data Our roadmap And we plan to share it in reverse order

7. Components of canonical JNK pathway in dorsal closure are required for wound closure MAP4K MAP3K MAP2K MAPK Txn factors 100 80 60 40 20 15 0 % open wounds Wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥30 N = lethal cross † genes If you don’t explain chart features BEFORE displaying the data, expect to lose half your audience

8. Components of canonical JNK pathway in dorsal closure are required for wound closure MAP4K MAP3K MAP2K MAPK Txn factors 100 80 60 40 20 15 0 % open wounds control w 1118 Wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥30 N = lethal cross † Make a point

9. Components of canonical JNK pathway in dorsal closure are required for wound closure MAP4K MAP3K MAP2K MAPK Txn factors 100 80 60 40 20 15 0 % open wounds control w 1118 † DJun DFos Wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥30 N = lethal cross † Make a point

10. Components of canonical JNK pathway in dorsal closure are required for wound closure MAP4K MAP3K MAP2K MAPK Txn factors 100 80 60 40 20 15 0 % open wounds control w 1118 † DJun DFosbsk X2 Wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥30 N = lethal cross † Make a point

11. Components of canonical JNK pathway in dorsal closure are required for wound closure MAP4K MAP3K MAP2K MAPK Txn factors 100 80 60 40 20 15 0 % open wounds control w 1118 † DJun DFosbsk X2 hep X2 Mkk4 Wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥30 N = lethal cross † Make a point

12. Components of canonical JNK pathway in dorsal closure are required for wound closure MAP4K MAP3K MAP2K MAPK Txn factors 100 80 60 40 20 15 0 % open wounds control w 1118 † DJun DFos slpr bsk X2 hep X2 Mkk4 Mekk1 Tak1 X2 Takl1 Takl2 Pk92B Wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥30 N = lethal cross † Make a point

13. Wound reporter: E22c-Gal4 A58-Gal4 Dor-2; A58-Gal4 N = ≥30 N = lethal cross † Components of canonical JNK pathway in dorsal closure are required for wound closure MAP4K MAP3K MAP2K MAPK Txn factors 100 80 60 40 20 15 0 % open wounds † msn X2 control w 1118 † DJun DFos slpr bsk X2 hep X2 Mkk4 Mekk1 Tak1 X2 Takl1 Takl2 Pk92B Make a point

14. More tips > www.stevetoms.net/powerpoint.htm Steve Toms, U.T. Communications Coach 713-662-6040 | steve@stevetoms.net Hit Space Bar or “Escape” to end Components of canonical JNK pathway in dorsal closure are required for wound closure MAP4K MAP3K MAP2K MAPK Txn factors 100 80 60 40 20 15 0 % open wounds † msn X2 control w 1118 † DJun DFos slpr bsk X2 hep X2 Mkk4Mekk1 Tak1 X2 Takl1 Takl2 Pk92B Wound reporter: E22c-Gal4 A58-Gal4 Dor-2; A58-Gal4 N = ≥30 N = lethal cross † Follow these guidelines; keep audiences turned on Purposeful builds keep the audience tuned in Graphic boxes move the eye to the next point > left-to-right Animations and transitions focus on the point being made Axis labels replace legends