1. Lecture 10 Web: pollev.com/ucibio Text: To: 37607 Type in: 169964

2. K m, V max, V 0 – What do they all mean? Parameters for enzyme action Rates and regulation!

3. 1. K m [S] at which rate is ½ of V Max NOT ________ of enzyme for substrate High K m = ?

4. Physiological relevance of K M Coordination of metabolism: Glucose  Energy Glucose  Glycogen First step in both = Glc  Glc-6-PO 4 Some tissues store, others need energy Convert Glc  Glycogen only when [Glc] high?

5. 2. V Max Compare rates of reaction at given [S] Some idea of efficacy of enzyme Regulation

6. 3. k Cat Also an idea of rate #molecules converted per second Time per conversion

7. Enzyme regulation Structurally, how can we regulate enzymes?

8. Enzyme regulation: Terms Allosteric Homotropic Heterotropic Reversible Irreversible

9. Competitive inhibition http://upload.wikimedia.org/wikipedia/commons/thumb/f/fb/Comp_inhib.svg/800px-Comp_inhib.svg.png

10. Allosteric noncompetitive inhibitor http://upload.wikimedia.org/wikipedia/commons/thumb/5/55/Non-competitive_inhibition.svg/536px-Non-competitive_inhibition.svg.png

11. Allosteric competitive inhibitor http://upload.wikimedia.org/wikipedia/commons/thumb/0/0f/Allosteric_comp_inhib_1.svg/800px-Allosteric_comp_inhib_1.svg.png

12. Allosteric competitive inhibitor http://upload.wikimedia.org/wikipedia/commons/thumb/a/a2/Allosteric_comp_inhib_2.svg/800px-Allosteric_comp_inhib_2.svg.png