1. BME 130 – Genomes Lecture 19 The histone code

2. Figure 7.1 Genomes 3 (© Garland Science 2007)

3. Figure 10.13 Genomes 3 (© Garland Science 2007) H3 H4 H2A H2B

4. CHiP-Seq DNA DNA-binding proteins (histones, e.g) crosslink Immuno-precipitate and fragment DNA Immuno-precipitate and fragment DNA Reverse cross-links and sequence

5. ChIP-Seq and ChIP-chip comparison of two histone marks Lower background & tighter peaks in ChIP-Seq (better contrast) trxG (activation) PcG (repression)

6. Promoter classes high CpG gene low CpG intermediate CpG CpG Highly expressed, housekeeping genes; other genes N=11,410 Genes with high tissue-specificity N=3,014 Mixture of genes N=3,338

7. NPC=neural progenitor cellsMEF=embryonic fibroblastsES = embryonic stem cells

8. Highly dynamic histone modification during differentiation

10. H3K36me3 marks gene bodies (may prevent aberrant transcriptional initiation)

11. Imprinting is reflected in H3K36me3 state

12. Nucleosome positioning and gene structure

17. Histone modification in cell division Haspin H3 P P Survivin Chromosomal passenger complex AuroraB (kinase)