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DArT-based linkage map of the
Solanum tuberosum ssp andigena accession LOP-868 (CIP ) Cultivar Name : Alca Tarma Chromosomes I to XII Maria V. Caraza Salas Elisa J. Mihovilovich Castro Mariela Aponte Villadoma Merideth Bonierbale
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Notes Population size =174 dihaploid progenies derived from haploidization of LOP 868 with haploid inducer lines IVP-101 and of Solanum phureja Juz. et Bukasov Total DArT markers mapped = 521 Mean DArT markers per chromosome = 43 Number of SSR markers mapped = 45 Mean SSR markers per chromosome = 4 Additionally: A major gene denoted Rladg for resistance to PLRV identified in this accession mapped on chromosome V (Velásquez et al. 2007) An AFLP marker P13M mapped close to the major gene for PLRV resistance Position of QTL for late blight resistance identified by association mapping in a Solanum t. andigena-derived B1 Population on chromosomes I, II, III; and IX Total length of the tetraploid map = cM
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Linkage map construction
The genetic map was constructed using the statistical program TetraploidMap (Hackett and Luo 2003) Marker order was established by the simulated annealing algorithm of TetraploidMap at starting T = 10, a cooling factor of = 0.85 and M = 100n random changes at each T° Chromosome assignment and polarity was established by mapping SSR markers and using “sol genomics network” maps; Potato meta-QTL consensus map and Solanum phureja diploid map 2010, as reference
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References Hackett CA, Luo ZW (2003) TetraploidMap: construction of a linkage map in autotetraploid species. J Hered 94(4):358–359 Velásquez AC, Mihovilovich E, Bonierbale M. (2007) Genetic characterization and mapping of major gene resistance to potato leafroll virus in Solanum tuberosum ssp. andigena. Theor Appl Genet 114 (6):
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Chromosome I QTL for late blight resistance found in B1 population by association mapping
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Chromosome II QTL for late blight resistance found in B1 population by association mapping
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Chromosome III QTL for late blight resistance found in B1 population by association mapping
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Chromosome IV
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Chromosome V
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Chromosome VI
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Chromosome VII
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Chromosome VIII
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Chromosome IX QTL for late blight resistance found in B1 population by association mapping
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Chromosome X
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Chromosome XI
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Chromosome XII
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