1. Rein et al., 2002 Current Biology The mushroom bodies (MBs) in the adult Drosophila brain

2. 1 neuroblast 3 types of neurons -  -  ’  ’ -  2 x 4 neuroblats 2 x 2000 neurons2 x 1 MB brain = central brain + optic lobes = 200.000 neurons central brain = central complex + MB central complex = 20.000 neurons 2 X MB = 2 X 2.000 neurons = 4000 neurons

3. midline  horizontal branch Srausfeld et al., MRT 2003 10  m A wild-type  neuron  vertical branch 10  m  lobe  lobe

4. Parental cell Mitotic recombination After DNA replication Two distinct mosaic progeny x x x FRT Mutant Centro mere Repressor x protein x Repressible marker x x x x x Marking mutant clones in a mosaic organism by the MARCM technique NB: one can make clones in wild-type background also!: visualization clones

5. Nb G N N FLP A multi-cellular Nb clone Two cell clones FLP Two mutually exclusive types of marked clones. Either all postmitotic neurons generated subsequently in the same lineage will be labeled (upper), or only two neurons derived from the GMC will be labeled in the whole lineage (lower).

6. In addition, mitotic recombination in a dividing GMC can generate a single cell clone Single-cell clone FLP

7. Twin spot MARCM to reveal the developmental origin and identity of neurons Yu et al., Nature Neursc. 2009 Review by Kao and Lee, COiN 2010 UAS-gfp-Mir and UAS-Cd2-Mir that silence the reporters UAS-Cd8::gfp and UAS-Cd2::rfp respectively.

8. Yu et al., Nature Neursc. 2009

9. -/- +/- cell-autonomous MARCM

10. NHL L3 0h APF Ecdysone pics during development

11. Summary of the mushroom body development

12. Axon reorganization of  neurons during metamorphosis. Clones were generated in NHL and examined 12, 18 and 24 hours APF

13. Lee et al., Neuron (2000) 28:807-818 Cell-autonomous requirement of the USP/EcR-B ecdysone receptor for mushroom body neuronal remodeling in Drosophila Zheng et al., Cell (2003) 112:303-315 TGF-  signaling activates steroid hormone receptor expression during neuronal remodeling in the Drosophila brain Boulanger et al., Nature Neuroscience (2011) 14(1):37-44 ftz-f1 and Hr39 opposing roles on EcR expression during Drosophila mushroom body neuron remodeling

15. and neuroblast clones also Summary of the genetic crosses for the MARCM-based genetic screen

16. Identification of the l(X)48 mutant defective in the pruning of larval-specific axons and dentrites

18. l(X)48 = usp 5, usp 4 and usp 3 two previously identified usp alleles. All three alleles result in changes of invariant arginines that contact phosphates in target DNA

19. Nuclear hormone receptors are ligand-dependent transcription factors

20. Nuclear receptors share common structure/function domains

21. A: The ECR-USP heterodimer binds ecdysone (E) and an EcRE in the DNA, activating a downstream promoter (arrow) B, C and D: Several models for negative regulation of ecdysone signaling pathways.

23. BR-C, E74 and E75 primary response genes for ecdysone are not individually essential for MB neuronal remodeling. Therefore, the USP/EcR-B heterodimer probably mediates the ecdysone-dependent MB neuronal remodeling through other target genes.

25. l(X)MB388 (dSmad2 1 ) carries a missense mutation in the dSmad2 gene and expression of wild-type dSmad2 cDNA rescues neuronal remodeling defects in l(X)MB388 mutant MB neurons.

27. EcR-A: no rescue, EcR-B1: partial rescue, EcR-B2: no rescue

29. THE ECDYSONE REGULATED GENE CASCADE King-Jones et al., Cell 2005

30. Caractéristiques de FTZ-F1 Nuclear steroid hormone receptor. Isolated in a biochemical screen for embryonic proteins binding regulatory sequences of ftz (Ueda et al Genes and Dev 90) and Alcohol dehydrogenase (Ayer et al Nucl Ac Res, 93). 2 mARN, 2 proteins that differ in N-terminal:  1043 aa in early embryogenesis.  816 aa from late embryo to pre-pupae. The nuclear receptor FTZ-F1

31.  FTZ-F1 is required for  neuron pruning. The expression of GAL4-201Y-driven GFP (green) and FASII (red) is shown in adult  neurons. Clones were induced at NHL

32. The nuclear receptors FTZ-F1 and HR39 LIGAND ADN 1 376 441 588 701 DN 1 510 575 803 1027 1043 22% 63 % HR39 FTZ-F1 1 510 575 803 LIGAND ADN

33. Both proteins have the same target sequences in vitro. Competition between the two receptors for binding to a common DNA element (Ohno et al., MCB 94). Antagonist role of the two proteins HR39 et FTZ-F1 in vivo? The nuclear receptors FTZ-F1 and HR39

34. HR39 ectopic expression blocks  neuron remodeling

35. g: Molecular map of the Hr39 locus with the intron/exon structure of the two main categories of mRNAs h: Expression of HR39 detected by western blot of adult heads. Controls (+/+) and mutant (Hr39 C105 /Def(2L))

36. Hr39 is required for normal  neuron development but not for  neuron pruning

37.  neuron pruning + ftz-f1 - Hr39 control P Hr39 -/- P P Hr39 -/- ;UAS-ftz-f1 + P P clones: Phenotype predicted obtained

38. ECR-B1  neuron pruning + ftz-f1 1 - Hr39 clones: Phenotype predicted obtained usp -/- UP usp -/- ;UAS-ftz-f1 + PUP usp -/- ; Hr39 -/- PUP usp -/- ; Hr39 -/- ;UAS-ftz-f1 + PUP

39. ECR-B1  neuron pruning + ftz-f1 - FTZ-F1 is binding the polytene chromosome band 42 A (EcR) (Lavorgna et al PNAS 1993) - at 10h APF reduced expression of EcR in hs-ftz-f1 RNAi (Lam and Thummel Cu.Bio. 2000) HYPOTHESIS 1 2 EcR-B1  neuron pruning + FTZ-F1 - Hr39 - HR39 surexp

40. Expression of ECR-B1 depends on normal FTZ-F1 activity in  neurons (a, b): wild-type, (c, d): ftz-f1 -/-, e): Quantification of ECR-B1 signal in arbitrary units (A.U.)

41. Expression of ECR-B1 depends on lack of HR39 activity in  neurons (f, g): wild-type, (h, i): + UAS-Hr39, (j): Quantification of ECR-B1 signal in arbitrary units (A.U.)

42. clones: Phenotype predicted obtained ftz-f1-/- UP ftz-f1-/-; UAS-EcRB1 + P P UAS-Hr39 + UP UAS-Hr39 + ;UAS-EcRB1 + P P UAS-Hr39 + ;UAS-ftz-f1 + P + UP in vivo competition 2 EcR-B1  neuron pruning + FTZ-F1 - HR39 surexp

43. in vivo competition between HR39 and  FTZ-F1 for  neuron remodeling

44. Zheng et al., Cell 2003  neuron pruning + ftz-f1 - Hr39 TGF-  /babo signaling EcR-B1 hypothesis: +

45. clones: Phenotype predicted obtained babo -/- UP babo -/- ; UAS-ftzf1 + P UP Hr39 -/- babo -/- P UP Hr39 -/- babo -/- ; UAS-ftzf1 + P UP Is ftz-f1/Hr39 pathway independent of TGF-  signaling?

46. Over-expression of HR39 and FTZ-F1 does not depend on babo activity in  neurons UAS-babo-deltaI = BABO dominant negative form UAS-babo = activated form of BABO

47. What insures Hr39 repression in the  neurons? ftz-f1 itself HR39 was overexpressed (1.78x) in ftz-f1 -/- clones when compared with wild-type clones.

48. Nuclear Receptor pathway TGF-  pathway ftz-f1 TGF-  / babo EcR-B1 Hr39 HR39 + USP and ecdysone Neuronal remodeling []

49. ChiP seq technique

50. In vivo binding of FTZ-F1 upstream of EcR-B1 transcription start site

51. Awasaki and Lee after Boulanger at al., Nature Neuroscience 2011

52. Thank you for your attention!