2. Enzymes
DEMO

3. Metabolism Reactions in your body
Body would burn up to get the amount of energy needed ( for molecules to collide with enough energy for reaction to occur)

4. What are Enzymes? Protein catalyst Act on substrates (reactants)
End in ASE
Ex. Lactase digest lactose
HOW?

5. Lower “Activation Energy”

6. Enzymes Without Enzyme With Enzyme Free Energy
Progress of the reaction
Reactants
Products
Free energy of activation

7. Enzyme Substrate Complex

8. Enzyme Substrate Complex

9. Enzymes are Specific!
Active Site
Induced Fit

10. Enzymes can be REUSED
Product is release

11. A product can be used as a substrate for another enzyme
E1 E2 E3 E4 E5 E6 A  B  C  D  E  F  G
NOT ON TEST!

12. Factors Effecting Enzymatic Speed
Temperature pH
Substrate concentration
Enzyme Concentration
WILL DISCUSS these 2
WHEN WE GET
BACK
(IN OUR LAB)

13. Temperature
Enzymes are PROTEIN
Heat too high they can denature

14. pH
Enzymes work best at an ‘optimal’ pH
Proteins can denature

15. Substrate Concentration
The more substrate the more reaction
Once all of the enzymes are being used the reaction cannot increase
Lab when we get back – skip

16. Enzyme Concentration Control by phosphorylation
Activate Add Phosphate (energy from ATP)
Lab when we get back- skip

17. Enzyme Inhibition Competitive Noncompetitive SKIP
EI- Substrate can’t get to active site
Comepitive- inhibitor binds to active site
Noncompetitive-inhibitor binds to ALLOSTERIC SITE

18. Feedback Inhibition
Activity of enzyme can be regulated by its end product
SKIP!!!

19. Enzyme Cofactors Help enzymes function Inorganic Organic- Coenzymes
Copper, Zinc, Iron
Organic- Coenzymes
Vitamins

20. Pellagra
Lack vitamin B3
pyrrolase

21. Scurvy Enzymes that impairs wound healing
Lack vitamin C- ships- throw over board- pick them up