2. What will I be learning today? Knowledge Plants have many different types of specialised cell Plant cells have similar features Investigation skills Using / focusing a microscope Making up your own slides Interpreting what you see

3. African Violet Looking at some specialised cells using microscopes African violet : Saintpaulia

4. Preparing a sample to investigate Take a small sample from underneath a leaf Mount on a microscope slide in a drop of water Care with scalpels and glass

5. Stomata: what you can expect to see The pink pigment is in some of the cells It is not in the guard cells (or subsidiary cells) so they look white Stoma (pore) Subsidiary Cell Guard cell

6. Close up on guard cells Find a clear stoma Identify the guard cells Use x10 or x40 objective lens Chloroplasts should be clearly visible chloroplasts

7. Trichomes (hairs) Made of several cells Form a physical barrier May produce chemicals May help conserve water Air bubble Trichomes

8. Close up on trichomes Find a big cell that’s part of a trichome Focus it on the highest magnification that you can Watch closely – you may see the cell contents moving

9. Other specialised cells Take thin slices along leaf veins Or along leaf petioles You may see spirals of lignin that strengthen xylem cells chloroplasts Spiral lignin in xylem vessels

10. Pollen Set up a microscope slide with a drop of water on it Remove a yellow anther from a flower Crush a section of the anther into the water using the blunt end of a dissecting needle or tweezers Cover with a cover slip Pollen grains

11. Petal Cells Prepare a slide with a drop of water Tear a petal to expose a ragged thin edge Place slices of the thin edge into the water on the slide Place a cover slip over the petal tissue Torn thin edge

12. Petal cells Dome shaped cells Maybe to allow pollinators to get a better grip on the petals*

13. Leaf Structure Take thin cross sections through a leaf Put these into a drop of water on a microscope slide Look for thin areas under the microscope to investigate the different tissues t t p m s u l

14. Successful learning today Skills -Did you learn any new skills today? -What skills were they? -If not – why do you think that was? Knowledge - What are hairs called on plants? -Can you name more specialised cells in plants? -Which cells that you have seen have chloroplasts? -What else have you learned?

15. Follow on work Building on the structures that students have seen, follow- on lessons could be developed to investigate: The function of guard cells –experiments with long balloons and sellotape to show curving of the balloon during inflation when one side has been taped The role of chloroplasts in photosynthesis –why doesn’t onion epidermis have chloroplasts? –why are they green? (pondweed stops photosynthesising in green light) The role of xylem –why do xylem vessels need strengthening with lignin? The structure of leaves / leaf tissues The function of trichomes –as a research / extension project