1. You start with a biologically relevant protein from a pathogen (Bacterium, virus, parasite…)

2. Reverse translate to obtain the DNA sequence

3. Our online-tool will electronically dissect the DNA sequence into peptides Define peptide length and overlap as required

4. Our online-tool will electronically dissect the DNA sequence into peptides Define peptide length and overlap as required

5. Our online-tool will electronically dissect the DNA sequence into peptides Define peptide length and overlap as required

6. Synthesize host constructs Amplify plasmids Digest to obtain single peptides Clone into expression vector Express

7. Synthesize host constructs Amplify plasmids Digest to obtain single peptides Clone into expression vector Express

8. Synthesize host constructs Amplify plasmids Digest to obtain single peptides Clone into expression vector Express

9. Synthesize host constructs Amplify plasmids Digest to obtain single peptides Clone into expression vector Express

10. Apply