1. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Fundamentals of Anatomy & Physiology SIXTH EDITION Frederic H. Martini Lecture 27: Fluid, Electrolyte, and Acid-Base Balance Lecturer: Dr. Barjis Room: P313 Phone: (718) 260-5285 E-Mail: ibarjis@citytech.cuny.edu

2. Learning Objectives Explain what is meant by “fluid balance,” “electrolyte balance,” and “acid-base balance” Compare the compositions of intracellular and extracellular fluids Identify the hormones that play important roles in regulating fluid and electrolyte balance Describe the movement of fluid that takes place within the ECF, between the ECF and the ICF, and between the ECF and the environment

3. Learning Objectives Discuss how sodium, potassium, calcium and chloride ions are regulated to maintain electrolyte balance Explain the buffering systems that balance the pH of the intracellular and extracellular fluids Describe the compensatory mechanisms involved in acid-base balance

4. Maintenance of normal fluid volume and composition is vital Extracellular fluid (ECF) includes: Interstitial fluid, plasma, lymph and other body fluids Intracellular fluid (ICF) includes: The cytosol (fluid inside the cell) Makes up about two-thirds of the total body water

5. Fluid balance Fluid is in a balance when the amount of water gained (e.g. through the digestive system) each day equals the amount of fluid lost (e.g. through urinary system, sweat glands) each day Electrolyte balance The ion gain each day equals the ion loss Electrolytes are ions released through the dissociation of inorganic compounds Acid-base balance H + gain is offset by their loss When acid–base balance exists, the pH of body fluids remains within normal limits (7.35-7.45). Fluid and electrolyte balance

6. The Composition of the Human Body

7. Homeostatic mechanisms respond to changes in ECF No receptors directly monitor fluid or electrolyte balance. However fluid and elctrolyte can be monitored by responding to changes in plasma volume or osmotic concentrations All water moves passively in response to osmotic gradients Body content of water or electrolytes rises if intake exceeds outflow Regulation of fluids and electrolytes

8. Cations in Body Fluids Major cations inside the cell (ICF) include Sodium (Na) Potassium (K) Magnesium (Mg) Major cations outside the cell (ECF) include Sodium (Na) Potassium (K) Calcisum (Ca) The concentration of cations inside the cell and outside the cell differs as shown in figure on the right e.g. there is much higher concentration of potassium in the ICF than in the ECF, there is much higher concentration of sodium in the ECF than in the ICF.

9. Anions in Body Fluids Major Anions inside the cell (ICF) include Chloride (Cl) Proteins Phosphates (HPO4) Bicarbonate (HCO3) SO4 Major Anions outside the cell (ECF) include Chloride (Cl) Proteins Phosphates (HPO4) Bicarbonate (HCO3) SO4 The concentration of anions inside the cell and outside the cell differs as shown in figure on the right e.g. there is much higher concentration of proteins and Phosphate in the ICF than in the ECF, there is much higher concentration of chloride in the ECF than in the ICF.

10. Despite the differences in the concentration of specific substances, the ICF and ECF osmotic concentrations are identical If the cell membrane were freely permeable, diffusion would continue until these ions were evenly distributed across the membrane Cations and Anions in Body Fluids

11. Fluid balance and electrolyte balance are mediated by three hormones: Antidiuretic hormone (ADH) Stimulates water conservation and the thirst center Aldosterone Controls Na + absorption and K + loss along the DCT Natriuretic peptides (ANP and BNP) Reduce thirst and block the release of ADH and aldosterone Primary regulatory hormones

12. Fluid moves freely within ECF compartment Water, electrolytes, proteins, fats and other nutrients and molceules are normally balanced by gains via Eating Drinking Metabolic generation Losses of water and other molcules are by: Urine Respiratory losses Perspiration Fecal Loss Fluid movement within the ECF

13. Fluid Exchanges

14. Hyponatremia Na + concentration in the ECF is reduced (overhydration) Hypernatremia Na + in the ECF is abnormally high Dehydration Develops when water loss outpaces water gains Water excess and depletion

15. Water movement between ECF and ICF If ECF becomes hypertonic relative to ICF, water moves from ICF to ECF If ECF becomes hypotonic relative to ICF, mater moves from ECF into cells Fluid shifts

16. Problems with Electrolyte Balance Usually result from sodium ion imbalances Potassium imbalances are less common, but more dangerous

17. Rate of sodium uptake across digestive tract directly proportional to dietary intake Sodium losses occur through urine and perspiration Shifts in sodium balance result in expansion or contraction of ECF Large variations corrected by homeostatic mechanisms Too low, ADH / aldosterone secreted Too high, ANP secreted Sodium balance

18. The Homeostatic Regulation of Normal Sodium Ion Concentrations in Body Fluids

19. The Integration of Fluid Volume Regulation and Sodium Ion Concentrations in Body Fluids

20. Potassium ion concentrations in ECF are low Not as closely regulated as sodium Potassium ion excretion increases as ECF concentrations rise Aldosterone secreted pH rises Potassium retention occurs when pH falls Potassium balance

21. Calcium balance Bone reserves, absorption in the digestive tract, and loss at kidneys Magnesium balance Absorbed by the PCT to keep pace with urinary losses ECF Concentrations of other electrolytes

22. The pH of the ECF remains between 7.35 and 7.45 If plasma levels fall below 7.35 (acidemia), acidosis results If plasma levels rise above 7.45 (alkalemia), alkalosis results Alteration outside these boundaries affects all body systems e.g. can result in coma, cardiac failure, and circulatory collapse Acid-base Balance The importance of pH control

23. Carbonic acid is most important factor affecting pH of ECF CO 2 reacts with water to form carbonic acid Inverse relationship between pH and concentration of CO 2 Common Acids

24. Buffer system consists of a weak acid and its anion Three major buffering systems: Protein buffer system Amino acid Hemoglobin buffer system H + are buffered by hemoglobin Carbonic acid-bicarbonate Buffers changes caused by organic and fixed acids Mechanisms of pH control

25. If pH climbs, the carboxyl group of amino acid acts as a weak acid If the pH drops, the amino group acts as a weak base Hemoglobin buffer system Prevents pH changes when P CO2 is rising or falling Protein buffer system

26. Carbonic acid-bicarbonate buffer system CO 2 + H 2 O  H 2 CO 3  H + + CO 3 – Has the following limitations: Cannot protect the ECF from pH changes due to increased or depressed CO 2 levels Only functions when respiratory system and control centers are working normally It is limited by availability of bicarbonate ions (bicarbonate reserve) Carbonic Acid-Bicarbonate Buffering System

27. The Carbonic Acid-Bicarbonate Buffer System

28. Lungs help regulate pH through carbonic acid - bicarbonate buffer system Changing respiratory rates changes P CO2 Respiratory compensation Kidneys help regulate pH through renal compensation Maintenance of acid-base balance

29. The Central Role of the Carbonic Acid- Bicarbonate Buffer System in the Regulation of Plasma pH

31. Reduced total body water content Impaired ability to perform renal compensation Increased water demands Reduced ability to concentrate urine Reduced sensitivity to ADH/ aldosterone Net loss of minerals Inability to perform respiratory compensation Secondary conditions that affect fluid, electrolyte, acid- base balance Changes with age include

32. What is meant by “fluid balance,” “electrolyte balance,” and “acid-base balance” The compositions of intracellular and extracellular fluids The hormones that play important roles in regulating fluid and electrolyte balance The movement of fluid that takes place within the ECF, between the ECF and the ICF, and between the ECF and the environment You should now be familiar with:

33. How sodium, potassium, calcium and chloride ions are regulated to maintain electrolyte balance The buffering systems that balance the pH of the intracellular and extracellular fluids The compensatory mechanisms involved in acid- base balance You should now be familiar with: