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Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程
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Agrobacterium tumefaciens and Crown galls
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Transformation of plant cells byAgrobacterium tumefaciens
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Agrobacteria cause crown gall tumors in plants
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Structure of the octopine and nopaline plasmids
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Structure of the nopaline Ti plasmid pTi C58 LB---left border RB---right border
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Map of the nopaline T-DNA region
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Formation of a cointegrate Ti plasmid
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Binary vector system baced on Bin19
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A GUS vector CaMV-cauliflower mosaic virus GUS-glucuronidase NPTII---neomycin phospho- trasferase II
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Regeneration of transgenic plants by leaf discs
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Regeneration of plants from protoplasts Auxins/cytokinins↑, Root development Auxins/cytokinins↓, Shoot development
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Regeneration of leaf disks infected by Agrobacterium
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Transfer of T-DNA from Agrobacterium in to a plant cell---Vir proteins and T-DNA transfer
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Transfering genes into plant cells by cointegration using T-DNA NPTII---neomycin phospho- trasferaseII
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Transfer of an antisense gene for polygalacturonase (PG) into tomato cells by using a binary vector CaMV-cauliflower mosaic virus GUS-glucuronidase
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Antisense technology
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The antisense RNA procedure for blocking or reducing the level of expression of a specific gene
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The regulation of biosynthesis of Ethylene (ETH)
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反义基因在耐储藏番茄育种中的应用 ACC gene ( 1- 氨基丙环烷羧酸氧化酶, 乙烯合成途径酶类 ) anti-sense ACC-mRNA ACC-mRNA 乙烯合成被抑制 (-) 5 3mRNA (-) 5 3 (+) 3 5 cDNA anti-sense ACC gene pCaMV35s-
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耐储藏番茄 华番一号 我国第一个基因工程农作物品种
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Systemic infection of a plant with recombinant geminivirus Vectors Gene A---Replication in plant cells Gene B---Infectivity
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Direct transfer of DNA into plant cells by microprojectile bombardement (shotgun)
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Stable transformation of plastids in tobacco plants Sp---spectinomycin Sm---streptomycin
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Cloning a plant gene by transposon tagging
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Cloning plant genes by T-DNA insertional Mutagenesis EMS---ethylmethane sulfonate
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Protection of plants from tobacco mosaic virus (TMV) infection by transgenic expression of the TMV coat protein(CP)
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Established Bt and Roundup ready crops
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Killing insect larvae with transgenic cotton plants that express the Bacillus thuringiensis(Bt) toxin gene
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苏云金芽胞杆菌( BT )毒素蛋白基因
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苏云金芽胞杆菌( BT )毒素蛋白基因 转 BT 基因水稻抗稻纵卷叶螟 抗病基因+抗虫基因+优质基因
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Engineering herbicide-resistance plants
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Roundup Ready® 转抗除草剂基因的 大豆和玉米
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Plants as bioreactors to produce antibodies
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