1. Development of Vaccine Approaches for Bovine Tuberculosis in Free- Ranging White-tailed Deer Mitchell Palmer, Ray Waters, Tyler Thacker National Animal Disease Center, ARS, USDA Ames, IA

2. Wildlife Vaccines Public health: rabies, plague, Lyme disease Livestock production: bovine TB, brucellosis, pseudorabies Iconic or endangered species: pasteurellosis (Bighorn sheep), anthrax (Black rhino)

3. Wildlife Vaccines -Challenges- Multiple species in natural transmission cycle Effective oral vaccine Safety of non-target species Fastidious feeding behavior Instability of vaccine in prevailing environmental conditions High reproductive rates and population turnover Requirement for low unit cost – Government funding (development, purchase, delivery)

4. Agent Mycobacterium tuberculosis complex > 99.95% sequence homology M. tuberculosis M. bovis – broadest host range M. caprae M. africanum M. canetti M. microti M. pinnipedii M. mungi

5. Cattle are often the original source of M. bovis in wildlife. As the prevalence of bovine tuberculosis decreases in livestock, the relative importance of wildlife reservoirs increases.

6. Tuberculosis Eradication 1900- tuberculosis leading cause of death – 10-25% of human tuberculosis due to M. bovis 1917- USDA eradication program – 15,000 deaths to M. bovis – 3x- number of foodborne deaths today Current surveillance is slaughter surveillance

7. Obstacles to Bovine Tuberculosis Eradication Importation of infected cattle – Need rapid, reliable and inexpensive test to at border crossings. Inability to Test and Remove Cattle – Need reliable, accurate tests to remove infected cattle without whole herd depopulation. Wildlife Reservoirs – Need for vaccines for wildlife. Safe vaccines Vaccine delivery systems – Need for vaccines for cattle. Infected vs Vaccinated

8. Tuberculosis in Wildlife Traditional eradication methods used for livestock DO NOT work with wildlife reservoirs. No country with an established wildlife reservoir of tuberculosis has successfully eradicated tuberculosis from domestic livestock. Vaccines viewed as possible control measure.

12. Mycobacterium bovis bacille Calmette-Guerín (BCG) BCG was developed 1906-1919. Originally isolated from a cow. Continuous passage led to decreased virulence Oldest of vaccines in use today. World’s most widely used vaccine (3 billion doses since 1920). Vaccinated individual may have positive skin test. Albert Calmette Camille Guerin

13. Vaccination in the US

14. Efficacy Does vaccination prevent infection? Does vaccination prevent disease (lesion development)? Does vaccination decrease transmission?

17. Medial retropharyngeal LN Total Score

18. Medial retropharyngeal LN BCG Danish: no lesions BCG Pasteur and non-vaccinates: Stages I-V Lung BCG Danish: Stages I-III BCG Pasteur: Stages I- III Non-vaccinates: Stages I-IV

19. Mediastinal LN Stage IStage IIStage IIIStage IVTotal Oral00000 Oral Bait00000 Parenteral4011318 Non-vaccinated1828 46 Lung Stage IStage IIStage IIIStage IVTotal Oral00000 Oral Bait00000 Parenteral1252625 Non-vaccinated68201549

20. Oral vaccination of white-tailed deer with M. bovis BCG VaccinatesNon-vaccinates Gross Lesions2/1711/16 Microscopic Lesions4/1711/16 M. bovis isolation5/179/16 Number of tissues822 Med retro LNIIIIIIIV Vaccinates9000 Non-vaccinates269511

21. Is it safe? Adverse reactions Non-target species Public health concerns – Possible exposure through venison consumption

25. Will vaccination of deer interfere with cattle testing? Possible cattle exposure to BCG False positive skin test results False positive IFN-gamma test results

26. Cattle (n=12): no conversion on skin test or Bovigam, all culture results negative Unvaccinated sentinel deer (n=10) BCG SC vaccinated deer (n=19)

27. 13 vaccinated deer 9 in-contact deer 7 calves 27 weeks of indirect contact No residual BCG No evidence of transmission of BCG from Vaccinated deer to cattle Immunological evidence of deer to deer transmission of BCG

28. Possums- NZ Badgers- UK, Ireland Wild boar- Spain White-tailed deer- US

29. Testing a molasses-based bait for oral vaccination of white- tailed deer (Odocoileus virginianus) against Mycobacterium bovis- M. R. Stafne, BS, Iowa State University, College of Veterinary Medicine Physical stability BCG Survivability Observations

30. Future Bait – Alternative Baits – Vaccine Delivery Mechanism – More field testing (attraction, stability, non-targets) Vaccine – Duration of immunity – Protection through secondary vaccination – Effect of high doses on protection Red tape – USDA, Michigan DNR, Michigan Department of Agriculture Approval for BCG in deer – Environmental impact statement, etc.