1. Presented by: Emily Campbell PI: Dr. Stephen Giovannoni Mentor: Paul Carini Marine Microbiology Lab http://giovannonilab.oregonstate.edu

2.  Discovered in 1990 by Stephen Giovannoni Most abundant heterotrophic organism in ocean surface waters world wide 1/3 of cells present in ocean surface water  Role of SAR11 Dissolved organic carbon cycling SAR11 oxidizes carbon → Releases CO 2  Ocean lacks large quantities of key nutrients CO 2 Organic Carbon AutotrophsHeterotrophs

3.  Most commonly found as phosphate (PO 4 )  An example of a nutrient found at low concentrations Below 5 nM  Required for all living things Nucleotides (DNA and RNA), phospholipids (cell membranes)  SAR11 thrives in low phosphate environments

4.  SAR11 isolate HTCC7211, HTCC1062, HTCC1002

5.  Hypothesis: Each SAR11 isolate can utilize different phosphorus compounds with varying abilities.  Prediction: If the isolate is able to utilize the given phosphorus-containing compound, we expect to observe maximum cell densities in excess of those cultures to which no phosphate has been added.

7. Negative ControlPositive ControlTest Compound Count Cells Plot Data

8.  2-aminoethylphosphate  cAMP  D-xylulose-phosphate  dATP  dCTP  dGTP  dTTP  D-Glucose 6 Phosphate  3-glycerophosphate  AMP  CMP  GMP  IMP  UMP  L-O Phosphoserine  Methylphosphate  Phosphite dibasic pentahydrate  Phosphoenol Pyruvate  Phosphonoacetic acid  Phytic Acid  D-ribose-5 phosphate  Sodium phosphite dibasic pentahydrate  Sodium Phosphonoformate  Sodium triphosphate  Uridine-5`- diphosphoglucose  DNA  RNA

12.  2-aminoethylphosphonic acid  dATP  dGTP  dCTP  dTTP  Deoxy-xylulose-phosphate  UMP  GMP  D-glucose 6 phosphate  3-glycerophosphate  Phosphoenol pyruvate  D-ribose-5 phosphate  Sodium phosphite dibasic pentahydrate  Sodium phosphonoformate HTCC 1062 HTCC 7211 HTCC 1002  2-aminoethylphosphonic acid  dGTP  dTTP  Deoxy-xylulose-phosphate  IMP  D-ribose-5 phosphate  Sodium phosphonoformate  Uridine 5’ diphosphoglucose  2-aminoethylphosphonic acid  dATP  dGTP  dCTP  dTTP  Deoxy-xylulose-phosphate  D-glucose 6 Phosphate  3-glycerophosphate  Methylphosphonic acid  Phosphoenol pyruvate  L-O Phosphoserine  D-ribose-5 phosphate  Sodium phosphite dibasic pentahydrate Shared between all three isolates Shared between HTCC1062 and HTCC7211 Shared between HTCC1062 and HTCC1002 Unique to that isolate

13. HTCC7211 HTCC1062 HTCC1002 IMP Uridine 5’ diphosphoglucose 2-aminoethylphosphonate dGTP dTTP D-xylulose-phosphate D-ribose-5 phosphate dATP dCTP D-glucose-6P 3-glycerophosphate PEP Phosphite Methylphosphonate L-O Phosphoserine UMP GMP Phosphonoformate

14.  Verify results Free phosphorus in stocks Test positive results in triplicate  What molecular pathways the compound could be used in What other necessities the phosphorus compound being transported could alleviate

15. Dr. Stephen Giovannoni Paul Carini HHMI Dr. Kevin Ahern