1. MICROBES Microscopic life forms that cannot be seen by the unaided eye May be plant or animal May be prokaryotic or eukaryotic

2. Importance of Microbes Pathogenic Help cure / prevent disease Break down organic matter to inorganic forms (decompose, recycle) –Autotrophs (inorganics) –Animals (organics) –Decomposers (inorganics, external digestion!)

3. Importance of Microbes Spoil foods Make / flavor foods Petroleum & Coal: unrecycled remnants Decay wooden structures Corrode iron & steel, damage concrete Food source Allow fouling community establishment

4. Classification of Microbes Plant vs Animal cell Cell Wall Chloroplasts Cell Nucleus Organelles/ Vacuoles Cytoplasm Cell Membrane

5. Classification of Microbes Prokaryotic vs Eukaryotic Nuclear Membrane

6. Classification of Microbes Prokaryotic Domain –Archaea Domain –Bacteria Domains and Kingdoms Eukaryotic Domain Eukarya –Protista –Fungi –Plantae –Animalia

8. Archaeabacteria [Prokaryotic] Methanococcus jannaschii –1 st archaea to be genetically sequenced

9. Bacteria [Prokaryotic] Blue-green algae –Cyanobacteria

10. Protista [Eukaryotic] Micro- & macro-algae

11. Fungi [Eukaryotic] Molds mycelia = ‘body’ hyphae = threads

12. Fungi [Eukaryotic] Molds

13. Molds (Ich, fin-tail rot) 2x

14. Fungi [Eukaryotic] Molds Yeast

15. Molds Yeast Fungi Fungi [Eukaryotic]

16. Bacteria Morphology (shapes) Coccus (cocci) spheres Bacillus (bacilli) rods Spirillus (spirilli) spiral, corkscrew (spirochetes)

17. Bacteria Morphology (shapes) Coccus (cocci) –diplococcus –streptococcus –staphylococcus Gonorrhea pneumonia Staph infections

18. Bacteria Morphology (shapes) Bacillus (bacilli) E. coli cholera Legionnaire’s disease meningitis

19. Bacteria Morphology (shapes) Bacillus (bacilli) salmonella peptic ulcers tuberculosis whooping cough

20. Bacteria Morphology (shapes) Spirillus (spirilli)

21. Growth (culture) of MICROBES Natural media –Extracts –Infusions Synthetic media –Nutrient broth –Nutrient agar

22. Broth Solidifiers Gelatin Animal protein Melts @ 20°C Digested by microbes Agar Plant carbohydrate Melts @ ~100°C, solidifies @ ~42°C NOT digested by (terrestrial) microbes Which is useful for incubation at human body temperature?