1. “ Sushi-gate 2008”: High School students apply DNA barcoding to fish sold in their NYC neighborhood, discover one-quarter is mislabeled Research report by students Kate Stoeckle and Louisa Strauss published in Pacific Fishing September 2008 Can DNA name this fish?

2. The New York Times August 21, 2008, page 1 Chosun Ilbo (Korean Daily News) circulation 2.2 million “Sushi-gate” draws wide interest CBS Early Show August 23, 2008

3. Barcode of Life Database (BOLD): www.barcodinglife.org BOLD project “Fish Market Survey” Analyzing Sushi-gate fish samples DNA sequencing at University of Guelph   1. Collect specimens2. Prepare samples3. Record data4. Send for analysis 5. Upload specimen, sequence data on BOLD 5. Search databases for identical or closely-related sequences 6. Learn more on FishBase, other  

4. Mislabelings were as more expensive or more desirable fish “Red Snapper” DNA ID: Nile PerchRed Snapper to scale Among 60 items tested: -Nile Perch (Africa), Lavender Jobfish (SE Asia), Slender Pinjalo (SE Asia), and Acadian Redfish (N Atlantic) sold as “Red Snapper” -Mozambique Tilapia sold as “White Tuna” -Smelt Roe sold as “Flying Fish Roe” -Caribbean Spotted Goatfish sold as “Mediterranean Red Mullet” -White Bass (farmed freshwater fish) sold as “Sea Bass” Mislabeled items were sold in 6 of 10 grocery stores/fish markets and 2 of 3 restaurants Range map: FishBase FishBase: D FlescherFishBase: John Casselman

5. The Trinity School Kate Stoeckle Louisa Strauss The Rockefeller University Jesse Ausubel Mark Stoeckle University of Guelph Robert Hanner Eugene Wong Fish Barcode of Life Initiative Unexplored territory? DNAHouse: 2009 student project exploring urban environment with DNA barcoding ?? Acknowledgments