1. Mining Mouse Vocalizations Jesin Zakaria Department of Computer Science and Engineering University of California Riverside

2. 124 Time (second) 125 40 kHz 100 laboratory mice Mouse Vocalizations Figure 1: top) A waveform of a sound sequence produced by a lab mouse, middle) A spectrogram of the sound, bottom) An idealized version of the spectrogram

3. A X Q X P A X C X P The intution behind symbolizing the spectrogram Figure 3: The two fragments of data shown in Figure 2.bottom aligned to produce the maximum overlap. (Best viewed in color) Figure 4: The data shown in Figure 2 augmented by labeled syllables Figure1: top ) Two 0.5 second spectrogram representations of fragments of the vocal output of a male mouse. bottom ) Idealized (by human intervention) versions of the above 2

4. 78 76.3Time (second) kHz 120 0 30 110 original idealized Background Figure 6: top) Original spectrogram, bottom) Idealized spectrogram (after thresholding and binarization) 3 1 1 1 4 4 8 8 7 Figure 7: left) A real spectrogram of a mouse vocalization can be approximated by samples of handwritten Farsi digits (right). Some Farsi digits were rotated or transposed to enhance the similarity 90.1 91.1 Time (sec) Figure 5: A snippet spectrogram that has seven syllables

5. I L SP connected components Figure 8: from left to right)snippet spectrogram, matrix corresponding to an idealized spectrogram I, matrix corresponding to the set of connected components L, mbrs of the candidate syllables Extracting syllables from spectrogram

6. I J K L M N O P A B C D E F G H a b c d e f g h i j k New Class Editing Ground truth I J K L M N O P A B C D E F G H Figure 9: Sixteen syllables provided by domain experts Figure 11: Ambiguity reduction of the original set of syllable classes. Representative examples from the reduced set of eleven classes are labeled as small letters

7. Editing Ground truth Figure 10: Thick/red curve represents the accuracy of classifying syllables of edited ground truth. Thin/blue curve represents the accuracy of classifying 692 labeled syllables using edited ground truth 0100200300400500600700 0 0.2 0.4 0.6 0.8 1 Adding more instances Classification Accuracy for edited ground truth for all the labeled syllables

8. Data mining Mouse Vocalizations ccccccgc eccccccc ecccccc ciaciaci dcibfcd ddcibfcd ccccccgc Figure 12: A clustering of eight snippets of mouse vocalization spectrograms using the string edit distance on the extracted syllables (spectrograms are rotated 90 degrees for visual clarity) Figure 13: A clustering of the same eight snippets of mouse vocalization shown in Figure 12 using the correlation method. The result appears near random Clustering mouse vocalizations

9. c c c c query image Data mining Mouse Vocalizations Similarity search / Query by content Figure 15: top) The query image from [2] was transcribed to cccc. Similar patterns are found in CT (first row) and KO (second row) mouse vocalizations in our collection Figure 14: top) A query image from [1], The syllable labels have been added by our algorithm to produce the query ciabqciacia, bottom) the two best matches found in our dataset; corresponding symbolic strings are ciafqcicia and ciqbqcaacja, with edit distance 2 and 3, respectively [1] J. M. S. Grimsley, et al., Development of Social Vocalizations in Mice. PLoS ONE 6(3): e17460 (2011). [2] T. E. Holy, Z. Guo, Ultrasonic songs of male mice, PLoS Biol 3(12): e386, (2005). query image c c c a a i b q a ii ciafqcicia Edit dist 2 ciqbqcaacja Edit dist 3

10. 944.7 – 945.2 sec 194.8 – 195.2 sec motif Data mining Mouse Vocalizations Assessing Motif Significance using z-score 16 17

11. Overrepresented in Control Overrepresented in Knock-out Figure 18: Examples of contrast set phrases. top) Three examples of a phrase ciacia that is overrepresented in KO, appearing 24 times in KO but never in CT. bottom) Two examples of a phrase dccccc that appears 39 times in CT and just twice in KO Contrast set mining using information gain