1. Nucleotide
Metabolism

2. nucleotide
nucleoside

5. a key molecule in purine and pyrimidine biosynthesis

6. purines: where does all them darn atoms come from??
fig 22-32

7. fig 22-33

8. fig 22-33

9. ETC.
fig 22-33, sorta

10. inosine, built
“on the ribose”
to make IMP
fig 22-33, still

11. and inosinate begat adenylate and xanthylate, and xanthylate
begat guanylate…
fig 22-34

12. regulation
of the
early steps
of purine
synthesis
fig 22-35

13. pyrimidine
biosynthesis
orotate, built
“off the ribose”
fig 22-36

14. pyrimidine
biosynthesis
OTP then
modified to
CTP, etc
fig 22-36

15. fig 22-38

16. 1) de novo synthesis (see above)
Two ways for mammals to make and/or
use purines and pyrimidines
1) de novo synthesis (see above)
2) salvage pathways
Ad + PRPP
AMP + PP

17. 1) de novo synthesis (see above)
Two ways for mammals to make and/or
use purines and pyrimidines
1) de novo synthesis (see above)
2) salvage pathways
hypoxanthine + PRPP
IMP + PP
Lesch-Nyhan disease

18. 1) de novo synthesis (see above)
Two ways for mammals to make and/or
use purines and pyrimidines
1) de novo synthesis (see above)
2) salvage pathways
pyrimidine salvage is similar but not well
understood in mammals…

19. inhibitors
of glutamine
amindo-
transferases
chemo-
therapeutics
fig 22-48

20. remember
the “D” in
DNA???
either or
fig 22-39

21. what’s the
difference
between
T and U??
fig 22-44

22. where the
THF gets
its methy...
fig 22-44

23. more chemotherapeutics

24. inhibitors of dihydrofolate reductase cycle
fig 22-49

25. catabolism of
purines…
xanthine
oxidase
fig 22-45

26. fig 22-45

27. out with gout!!!
1735

28. out with gout!!!
xanthine oxidase too active
fig 22-47

29. out with gout!!!
xanthine oxidase inhibitor
fig 22-47