1. www.WilksIR.com InfraCal TOG/TPH Analyzer, Model HATR-T2 Extraction Procedure

2. Collect a measured amount of sample Collect a measured amount of sample

3. For samples that will be stored for more than a day or for samples with a high pH: Adjust the pH to less than 2 with Hydrochloric acid or Sulfuric Acid (typically 3-5 drops depending on buffers in sample)

4. Add Solvent Add Solvent 10 parts sample to 1 part solvent For example: For 150 ml of sample add 15 ml of solvent

5. Add Solvent Add Solvent 10 parts sample to 1 part solvent For example: For 150 ml of sample add 15 ml of perchloroethylene

6. Shake for 2 minutes Shake for 2 minutes

7. Open the cap on the bottle during the 2 minute shaking to relieve the pressure Open the cap on the bottle during the 2 minute shaking to relieve the pressure

8. Hexane is lighter than water and will float on the top of the sample Hexane is lighter than water and will float on the top of the sample

9. Using a syringe or pipette, take 50 microliters of solvent extract Using a syringe or pipette, take 50 microliters of solvent extract

10. And deposit it in the center of the sample crystal And deposit it in the center of the sample crystal

11. Press the run button Press the run button

12. 14 Oil concentration displayed Oil concentration displayed

13. Silica Gel Extraction The difference between TPH (Total Petroleum Hydrocarbon) and TOG (Total Oil and Grease) is the polar organics are removed from the extract using silica gel. The remaining hydrocarbons are the non-polar components considered to be TPH

14. Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica

15. Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica Put funnel into bottle Put funnel into bottle Put solvent extract through Put solvent extract through silica gel

16. Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica Put funnel into bottle Put funnel into bottle Put solvent extract through Put solvent extract through silica gel Using a 50 microliter syringe or pipette deposit on the center of the sample plate crystal Using a 50 microliter syringe or pipette deposit on the center of the sample plate crystal

17. Press the run button Press the run button

18. 14 Oil concentration displayed Oil concentration displayed

19. For questions please contact us at: tech@WilksIR.com, or 203-855-9136 InfraCal TOG/TPH Analyzer Extraction Procedure