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Figure 5.1 Membrane Molecular Structure

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Presentation on theme: "Figure 5.1 Membrane Molecular Structure"— Presentation transcript:

1 Figure 5.1 Membrane Molecular Structure
Outside of cell Extracellular matrix Phospholipid Cytoskeleton Inside of cell

2 In-Text Art, Ch. 5, p. 64 “Head” “Tails”

3 Outside of cell (aqueous)
In-Text Art, Ch. 5, p. 65 Outside of cell (aqueous) Hydrophobic interior of bilayer Inside of cell (aqueous)

4 In-Text Art, Ch. 5, p. 66 Cells

5 Figure 5.2 Rapid Diffusion of Membrane Proteins (Part 1)
Proteins embedded in a membrane can diffuse freely within the membrane. Membrane proteins Mouse cell Human cell

6 Figure 5.2 Rapid Diffusion of Membrane Proteins (Part 2)
Proteins embedded in a membrane can diffuse freely within the membrane. Membrane proteins Mouse cell Human cell Heterokaryon Membrane proteins can diffuse rapidly in the plane of the membrane.

7 Figure 5.2 Rapid Diffusion of Membrane Proteins (Part 3)
The experiment was repeated at various temperatures with the following results: Cells with mixed proteins (%) Temperature (C) 15 20 26 8 42 77 Plot these data on a graph of Percentage Mixed vs. Temperature. Explain these data, relating the results to the concepts of diffusion and membrane fluidity.

8 Figure 5.3 Osmosis Can Modify the Shapes of Cells (Part 1)
Hypertonic on the outside (concentrated solutes outside) Isotonic (equivalent solute concentration) Hypotonic on the outside (dilute solutes outside) Inside of cell Outside of cell

9 Figure 5.3 Osmosis Can Modify the Shapes of Cells (Part 2)
Hypertonic on the outside (concentrated solutes outside) Isotonic (equivalent solute concentration) Hypotonic on the outside (dilute solutes outside) Animal cell (red blood cells)

10 Figure 5.3 Osmosis Can Modify the Shapes of Cells (Part 3)
Hypertonic on the outside (concentrated solutes outside) Isotonic (equivalent solute concentration) Hypotonic on the outside (dilute solutes outside) Plant cell (leaf epithelial cells)

11 Figure 5.4 A Ligand-Gated Channel Protein Opens in Response to a Stimulus
Outside of cell Stimulus molecule (ligand) Binding site Channel protein Hydrophobic interior of bilayer Hydrophilic pore Closed channel Inside of cell

12 Figure 5.5 Aquaporins Increase Membrane Permeability to Water (Part 1)
Aquaporin increases membrane permeability to water. Aquaporin mRNA Aquaporin channel Protein synthesis

13 Figure 5.5 Aquaporins Increase Membrane Permeability to Water (Part 2)
Aquaporin increases membrane permeability to water. Aquaporin mRNA Aquaporin channel Protein synthesis 3.5 minutes in hypotonic solution Aquaporin increases the rate of water diffusion across the cell membrane.

14 Figure 5.5 Aquaporins Increase Membrane Permeability to Water (Part 3)
Oocytes were injected with aquaporin mRNA (red circles) or a solution without mRNA (blue circles). Water permeability was tested by incubating the oocytes in hypotonic solution and measuring cell volume. After time X in the upper curve, intact oocytes were not visible: X With mRNA Without mRNA Relative volume Time (min) A. Why did the cells increase in volume? B. What happened at time X? C. Calculate the relative rates (volume increase per minute) of swelling in the control and experimental curves. What does this show about the effectiveness of mRNA injection?

15 Figure 5.6 A Carrier Protein Facilitates Diffusion (Part 1)
Outside of cell High glucose concentration Glucose Glucose carrier protein Inside of cell Low glucose concentration

16 Figure 5.6 A Carrier Protein Facilitates Diffusion (Part 2)
Rate of diffusion into the cell Glucose concentration outside the cell

17 Figure 5.7 Primary Active Transport: The Sodium–Potassium Pump
Outside of cell High Na+ concentration, low K+ concentration Na+ Na+– K+ pump K+ K+ ATP Pi Na+ Pi Pi ADP Pi K+ Inside of cell High K+ concentration, low Na+ concentration

18 Figure 5.8 Endocytosis and Exocytosis (Part 1)
(A) Endocytosis Outside of cell Plasma membrane Inside of cell Endocytotic vesicle

19 Figure 5.8 Endocytosis and Exocytosis (Part 2)
(B) Exocytosis Secretory vesicle

20 Figure 5.9 Receptor-Mediated Endocytosis (Part 1)
Cytoplasm Clathrin molecules Coated vesicle Outside of cell Specific substance binding to receptor proteins Coated pit 20

21 Figure 5.9 Receptor-Mediated Endocytosis (Part 2)
Outside of cell Specific substance binding to receptor proteins Coated pit Cytoplasm Coated vesicle Clathrin molecules 21

22 Figure 5.10 Chemical Signaling Concepts
Receptor Secreting cell Target cell Target cell Circulatory vessel (e.g., a blood vessel) Target cell 22

23 Figure 5.11 Signal Transduction Concepts
Signal molecule Receptor Short-term responses: enzyme activation, cell movement Inactive signal transduction molecule Activated signal transduction molecule Long-term responses: altered DNA transcription 23

24 Figure 5.12 A Signal Binds to Its Receptor
Ligand Outside of cell Cell membrane Inside of cell 24

25 In-Text Art, Ch. 5, p. 76 Signal molecule Receptor R + L RL 25

26 Figure 5.13 A Protein Kinase Receptor
Signal (insulin) Outside of cell Receptor Protein kinase domain (inactive) ATP ADP Phosphate groups Target Cellular responses Inside of cell 26

27 Figure 5.14 A G Protein–Linked Receptor (Part 1)
Outside of cell Signal (hormone) GDP G protein- linked receptor Inactive G protein Inactive effector protein Inside of cell 27

28 Figure 5.14 A G Protein–Linked Receptor (Part 2)
Outside of cell GTP Activated G protein Inside of cell 28

29 Figure 5.14 A G Protein–Linked Receptor (Part 3)
Outside of cell Activated effector protein GDP Product Reactant Amplification Inside of cell 29

30 Figure 5.15 The Discovery of a Second Messenger (Part 1)
A second messenger mediates between receptor activation at the plasma membrane and enzyme activation in the cytoplasm. Liver Cytoplasm contains inactive glycogen phos-phorylase Membranes contain epinephrine receptors 30

31 Figure 5.15 The Discovery of a Second Messenger (Part 2)
A second messenger mediates between receptor activation at the plasma membrane and enzyme activation in the cytoplasm. Active glycogen phosphorylase is present in the cytoplasm. A soluble second messenger, produced by hormone-activated membranes, is present in the solution and activates enzymes in the cytoplasm. The activity of previously inactive liver glycogen phosphorylase was measured with and without epinephrine incubation, with these results: Condition Enzyme activity (units) Homogenate Homogenate + epinephrine Cytoplasm fraction Cytoplasm + epinephrine Cytoplasm + membranes Cytoplasm + membranes + epinephrine 0.4 2.5 0.2 2.0 What do these data show? Propose an experiment to show that the factor that activates the enzyme is stable on heating and give predicted data. Propose an experiment to show that cAMP can replace the particulate fraction and hormone treatment and give predicted data. 31

32 Figure 5.16 The Formation of Cyclic AMP (Part 1)
Adenylyl cyclase cAMP + PPi ATP 32

33 Figure 5.16 The Formation of Cyclic AMP (Part 2)
Adenine Phosphate groups ATP 33

34 Figure 5.16 The Formation of Cyclic AMP (Part 3)
Cyclic AMP (cAMP) 34

35 Figure 5.17 A Cascade of Reactions Leads to Altered Enzyme Activity (Part 1)
Epinephrine Outside of cell Activated G protein subunit Epinephrine receptor Plasma membrane Activated adenylyl cyclase GTP ATP 20 cAMP Inactive protein kinase A Active glycogen synthase 20 Active protein kinase A Inactive glycogen synthase Inactive phosphorylase kinase 100 Active phosphorylase kinase 35

36 Active phosphorylase kinase
Figure A Cascade of Reactions Leads to Altered Enzyme Activity (Part 2) 100 Active phosphorylase kinase Inactive glycogen phosphorylase 1,000 Active glycogen phosphorylase Glycogen 10,000 Glucose 1-phosphate Glucose Inside of cell 10,000 Blood glucose Outside of cell 36

37 Figure 5.18 Signal Transduction Regulatory Mechanisms (Part 1)
ATP Protein kinase P Inactive enzyme Active enzyme Protein phosphatase Pi 37

38 Figure 5.18 Signal Transduction Regulatory Mechanisms (Part 2)
Receptor binding Inactive G protein Active G protein GDP GTP GTPase 38

39 Figure 5.18 Signal Transduction Regulatory Mechanisms (Part 3)
Adenylyl cyclase Phosphodiesterase ATP cAMP AMP 39

40 Figure 5.19 Caffeine and the Cell Membrane (Part 1)
Outside of cell Plasma membrane Inside of cell 40

41 Figure 5.19 Caffeine and the Cell Membrane (Part 2)
Adenosine 41


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