1. Diuretics and Dehydrants

2. §1 Diuretics

3. Abnormalities in fluid volume and electrolyte composition are common and important clinical problems. Drugs that block the transport functions of the renal tubules are valuable clinical tools in the treatment of these disorders. It was not until 1957 that a practical and powerful diuretic agent (chlorothiazide) became available for widespread use. Technically, the term "diuresis" signifies an increase in urine volume, while "natriuresis" denotes an increase in renal sodium excretion. Because natriuretic drugs almost always also increase water excretion, they are usually called diuretics.

4. Most diuretics act upon a single anatomic segment of the nephron. Because these segments have distinctive transport functions, the first section of this chapter is devoted to a review of those features of renal tubule physiology that are relevant to diuretic action.

5. Renal Tubule Transport Mechanisms

6. 1. Proximal tubule 2. thick ascending limb of Henle’s loop 2. thick ascending limb of Henle’s loop 3. Distal tubule and collecting ducts

7. Proximal Tubule acetazolamide

8. Loop of Henle loop diuretics

9. Distal Convoluted Tubule thiazide

10. Collecting Tubule Triamterene

11. ※ Common diuretics

12. Common diuretics exert effects on specific membrane transport proteins in renal tubular epithelial cells (loop diuretics, thiazides, amiloride, and triamterene) exert osmotic effects that prevent water reabsorption (mannitol) exert effects by inhibiting enzymes (acetazolamide) exert effects by interfering with hormone receptors in renal epithelial cells (spironolactone)

13. Ⅰ. High –ceiling diuretics (loop diuretics) Furosemide, Ethacrynic acid, Bumetanide

14. [ Pharmacological actions ]  Site of action: thick ascending limb of Henle’s loop  Mechanism: competing with Cl － for Cl － joint-part of Na + -K + -2Cl － cotransporter  Effects 1. Diuresis: Na +, K +, 2Cl －, Mg 2+, Ca 2+ excretion↑ 2.↑renal blood flow: ↓renal vescular resistance

15. pharmacokinetics 1. p.o. & intravenous administration 2. excretion: by organic acid secretion route in the proximal tubule uric acid ( 尿酸 ) ↑ gout indomethacin( 吲哚美辛 ) probenecid ( 丙磺舒 )

16. Clinical uses 1. Acute and Severe edema Acute pulmonary edema cerebral edema

17. Clinical uses 3. acute renal failure: early stage 4. Hypercalcemia : 5. Overdose of some toxicants: bromide, fluoride, iodide

18. Adverse reactions Adverse reactions 1. Electrolyte disorders : hypochloremia, hypomagnesemia, hypokalemia ※  CHF: ↑intoxication with digitalis  Hepatic cirrhosis: coma 2. Ototoxicity: dose-related hearing impairment: tinnitus, hearing loss, etc.

19. Adverse reactions 3. hyperuricemia (1) hypovolemia: reabsorption of uric acid (2) competing with diuretic for organic acid secretion route →secretion of uric acid↓ 4. Others: GI reactions, allergic reactions

20. Ⅱ.Moderate efficacy diuretics Thiazides: ※ Hydrochlorothiazide ( 氢氯噻嗪 ), chlorothiazide ( 氯噻嗪 ) Indapamide ( 吲哒帕胺 ) chlortalidone( 氯酞酮 )

21. Pharmacological actions  Site of action: proximal end of distal tubule  Mechanism : inhibit Na + -Cl － cotransporter

22. 1) Diuresis: Na +, K +, 2Cl －, Mg 2+ excretion↑ 2) ↑Ca 2+ reabsorption in distal tubules: primary hypercalciuria 3) reduced peripheral vascular resistance   Effects

23. Clinical uses 1. Chronic edema  mild to moderate cardiac edema: first choice  ascites( 腹水 )due to cirrhosis( 肝硬 变 )

24. 2. hypertension early stage: ↓ blood flow late stage : excretion of Na + ↑→ Na + -Ca 2+ exchange↓ → ↓Ca 2+ in cell → tension of arterial↓

25. 3. nephrogenic insipidus 1) ↓ PDE → intracellular cAMP↑→ water permeating the tubule↑ → reabsorption of water↑ 2) excretion of NaCl↑ → plasma osmotic pressure↓ → thirst feeling↓ → amount of drinking↓ → urine↓ 4. Primary hypercalciuria

26. Adverse reactions 1. electrolyte disorders 2. retention of uric acid and calcium 3. hyperglycemia and hyperlipidemia 4. allergic reaction:

27. Ⅲ. Potassium-sparing diuretics Site of action: distal tubule and collecting duct

28. aldosterone antagonist: Spironolactone (antisterone) 【 mechanism 】 : competing with ald. for ald-R in distal tubule and collecting duct → Na + -K + exchange↓ 【 characteristics 】 ※ ineffective in adrenalectomized animal ※ slow onset ＆ long duration

29. Clinical uses 1. primary hyperaldosteronism 2. liver cirrhosis and nephritis syndrome (1)inactivation of ald.↓ (2)circulation blood volume↓

30. Adverse reaction 1. hyperkalemia: renal insufficiency 2. endocrine abnormality: impotence, gynecomastia,

31. nonaldosterone antagonists ※ triameterene( 氨苯蝶啶 ) ※ amiloride ( 阿米洛利 ) Mechanism :Mechanism Block Na + –channel → ↓ Na + entry →K + secretion↓ clinical uses : adverse reaction :

32. §2 dehydrants Difficultly penetrate membrane filtrated by glomerulus easily Not be reabsorpted by renal tubules Not be metabolized

33. mannitol （甘露醇） mannitol （甘露醇） Pharmacological actions 1. dehydrant effect 2. diuretic effect

34. Cliniucal uses 1. Brain edema 2. Glaucoma 3. Prevent acute renal failure Adverse reaction extracellular volume expansion contraindication: chronic heart failure