1. Station No. Answer M Station No. AnswerM Station 11- Station 7 1- 2- Station 21- Station 8 1- 2- Station 31- Station 9 1- 2- Station 41- Station 10 1- 2- Station 51- Station 11 1- 2- Station 61- Station 12 1- 2- Sum/12Sum/12 OSPE/24/27 Check list/3 Bioinformatics/3 Total/30 marks Answer Sheet Name:………………………………… ID Number:………………………… Stations = 12 each station with 2 marks. Time: 12 min. each station 1 min. Check List …………………………………(3 marks):

2. Materials required Provided tomato slice -Extraction buffer -95-100% ice cold Ethyl Alcohol- Plastic knife -Watch glass-Nylon gauze-Funnel-Test tubes. Steps 1.Chop tomato slice into very fine pieces by your plastic knife. 2.Add 2ml of the extraction buffer to the chopped tomato and grind for 1 minute to a fine slurry in the watch glass. 3.Filter the previous cells solution into a test tube using nylon gauze and a funnel (to get fibers free solution). 4.Tilt your test tube containing the filtrate and slowly pour equal volume of 95-100% ice cold alcohol into the tube down the side of the test tube, 5.5-Observe at the layer between the tomato juice and alcohol you will see a whitish, "cotton like" substance (threads. This is the DNA. LEAVE the tube with Extract DNA in your RACK OutcomeExtract DNA. Name:………………………………… ID Number:………………………… Guide Notes For students to use during lab DNA extraction Activity (18min) Perform and Practice the proper way to Extract DNA from tomato.

3. Student ID-or sample number StepsY/N 1-Chop tomato slices into very fine pieces. (0.5) 2- Grinding:  Add 2ml of the extraction buffer (0.25)  Grind for 1 minute to a fine slurry in the watch glass. (0.25) 3 -Filter cells solution  Into a test tube (0.25)  using nylon gauze (0.25)  and a funnel (0.25) 4- Addition of Alcohol  Tilt test tube and slowly pour (0.25)  equal volume of 95-100% ice cold alcohol (0.25)  into the tube down the side of the test tube, (0.25) 5-Observe at the layer between the tomato juice and alcohol you will see a whitish, "cotton like" substance (threads. This is the DNA. (0.5) Evaluation (3 marks) Checklist of DNA Extraction(18min)