1. GeneLocation (bp ) PMA1 UAS 1 2 3 4 5 6 7 8 9 -926 ~ -677 -304 ~ -47 168 ~ 376 584 ~ 807 1010 ~ 1250 2018 ~ 2290 2866 ~ 3098 3287 ~ 3500 3448 ~ 3662 3619 ~ 3905 ADH1 UAS 1 2 3 -670 ~ -455 -235 ~ -13 844 ~ 1013 1231 ~ 1400 PYK1 UAS 1 2 3 4 5 -815 ~ -566 -288 ~ 19 508 ~ 799 1104 ~ 1372 1470 ~ 1720 1695 ~ 1995 5 6 7 9 Poly(A) 4 8 32 1 PMA1 (2.7 kb) TATA UAS +1 2 3 Poly(A) ADH1 (1.0 kb) 1 UAS TATA +1 1 4 5 2 3 Poly(A) PYK1 (1.5 kb) TATA UAS +1

2. Rap1 TBP Rpb1 INPUT * * * * PMA1ADH1 PYK1 123457 1 2 312345 UUU

3. Spt16 Spt5 Rtf1 INPUT Sub2 Rna14 * PMA1ADH1 PYK1 123457 1 2 312345 UUU PMA1ADH1PYK1 123457 1 2 312345 U U U INPUT * * * *