1. DEVELOPMENT OF A NEW APPARATUS FOR THE ASSESSMENT OF HEALTH IMPACT FROM EXPOSURE TO FIRE COMBUSTION PRODUCTS G. Chattopadhyay 1, Paul Hallahan 2, Paul Brockbank 3, F. Lestari 4, A. Hayes 4, A. Green 4 1 School of Civil and Environmental Engineering 2 School of Chemical sciences 3 School of Chemical Engineering and Industrial Chemistry 4 School of Safety Science The University of New South Wales, Sydney 2052, Australia

2. TECHNIQUES INCORPORATED IN THE APPARATUS Fire combustion dynamics measurement (TGA analysis) In vitro cytotoxicity measurement –Impinger –Direct exposure Sampling for chemical analysis GC-MS Chemical analysis –Thermal desorption –Cryo-focusing

3. TGA data Acc Flow control Load cell for TGA data Tube furnace Mixing chamber Basic components of the apparatus Frame

4. Tube Furnace Quartz tube 1000 mm long X 40 mm diameter

5. Load Cell for TGA data TGA data cable Gas inlet Bottom of the quartz Tube furnace Ground glass joint Fits onto the tube furnace Hole in polycarbonate Enclosure to tare balance Polycarbonate enclosure

6. Mixing Chamber Sampling lines Exhaust line from mixing chamber to lab suction system Exhaust line to lab suction system from Tube furnace enclosure Mixing Chamber : 310 (L) X 300(W) X 310 (H) Volume: 28.83 (L)

7. Flow control components Temperature control for Direct exposure cells Flow control for gas and sampling streams Impinger Suction pump for Collecting samples Temperature control For tube furnace

8. Air sampling using culture medium (serum free) 96 well plates MTS assay Experiment flowchart Impinger method

9. inlet outlet membrane Membrane preparation Snapwell insert Navicyte chamber MTS Assay Direct exposure at the air/liquid interface

10. Chemical analysis for combustion products Sorption in a solid sorbent ATD-GCMS

11. Tube Desorb Cold trap at -20° C To GC column transfer line

12. Trap Desorb To GC column transfer line

14. Preliminary result CO 2 Impinger method Direct exposure

15. PMMA cytotoxicity under non-flaming condition at different post exposure time

16. Potential applications Fire toxicity testing for materials or products Screening and early sign of fire hazard to fire fighters (biosensor) Toxicity studies for gases, particulates, non- hydrosoluble compounds, complex mixtures, and organic volatile compounds Many applications in odour analysis and odour management