1. Navy Medical Entomology Research in Southeast Asia LT Kathryn A. Barbara US Naval Medical Research Unit #2 Jakarta, Indonesia DoD Pest Management Workshop, Feb 8-12, 2010

2. Disclaimer The opinions or assertions expressed herein are the private views of the authors and are not to be construed as representing those of the US Navy, the Department of Defense, or the Cambodia, Singapore, or Indonesia Ministry of Health.

3. Effect of Land Use on Malaria Vector Diversity and Distribution in Pursat Province, Cambodia K. A. Barbara, S. Rusmiarto, R. Pudiantari, A. Farzeli, I. Nurlaila, and S. Sovannaroth 2 US Naval Research Unit No. 2 Jakarta, Indonesia 2 The National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia

4. Effect of Land Use on Malaria Vector Diversity and Distribution in Pursat Province, Cambodia Before 1970 over 73% of Cambodia was forest cover, currently 58% (70% primary forest in 1970, 3.1% currently) Rate of deforestation in Cambodia: –1990-2005: 19.3% lost = 2,499,00 hectares (444,000 hectares primary forest) –Annual Change 2000-2005: 218,800 ha Deforestation in Cambodia is driven by –agricultural development/expansion –road construction –mining –hydropower development –extensive commercial forest exploitation

5. Effect of Land Use on Malaria Vector Diversity and Distribution Conduct bionomics studies in areas bordering old forest growth and areas cleared of forest trees and reestablished as small agricultural areas Hypotheses: –Anopheles dirus will be the most abundant Anopheles spp. collected bordering the primary forest areas. –An. minimus (Theobald) will be the most abundant Anopheles spp. collected in the areas cleared for agricultural use. –An. dirus will be the main malaria vector in the forest sites; An. minimus will be the dominant malaria vector in the agriculture site.

6. Study Site

7. Mosquito Collections Collections were conducted from October 2008 to January 2009 Indoor/outdoor, 12h human landing collections (1800-0600) over 8 days 8 houses served as permanent collection sites 22 nights (agricultural site), 23 nights (forest site)

8. Forest Agricultural ~ 4km

9. Results AgriculturalForest Anopheles speciesIndoor (%) Outdoor (%) HBRIndoor (%)Outdoor (%)HBRTotal (%) An. barbirostris 254 (10.1)263 (8.2)2.9474 (3.8)135 (5.1)1.14726 (7.0) An. dirus 585 (23.3)795 (24.7)7.84*1234 (62.7)1473 (55.9)14.71*4087 (39.6) An. jamesi 97 (3.9)131 (4.1)1.305 (0.3)00.03233 (2.3) An. kochi 316 (12.6)363 (11.3)3.86143 (7.3)230 (8.7)2.031052 (10.2) An. maculatus 775 (30.9)1016(31.5)10.18*413 (21.0)590 (22.4)*5.45*2794 (27.0)* An. minimus 278 (11.1)444 (13.8)*4.1072 (3.7)147 (5.6)*1.19941 (9.1)* An. phillipinensis 123 (4.9)112 (3.5)1.3419 (1.0)37 (1.4)0.30291 (2.8) An. tesselatus 15 (0.6)5 (0.2)0.0601 (0.04)0.0121 (0.2) An. vagus 65 (2.6)92 (2.9)0.949 (0.5)20 (0.8)0.16186 (1.8) Total 2508322119692633*10331 HBR = mosquitoes/human/12 hour collection period * Significance at p < 0.05

10. Discussion An. dirus HBR highest in FOREST An. maculatus HBR highest in AGRICULTURE An. minimus more prevalent in agriculture site An. dirus prevalent mosquito in both locations –Forest mosquitoes occasionally collected at forest fringes –Highly anthropophilic- our results show no significant difference between indoor and outdoor collections –Very difficult to control

11. AgricultureForest Species Pv 210 or 247 PfMixed (Pf + Pv) Mixed (Pv210 + Pv247) EIRPv 210 or 247 PfMixed (Pf + Pv) Mixed (Pv210 + Pv247) EIR An. minimus s.l.10000.011000 An. dirus s.l.*24120.0500000 An. philipinensis0000000000 An. tesellatus0000000000 An. vagus0000000000 Pv = P. vivax Pf = P. falciparum An. dirus collected from forest not yet tested An. maculatus not yet tested ELISA Results (Preliminary)

12. PCR Results Anopheles minimus s.l. (Preliminary) Site An. minimus s.s. (Species A) An. harrisoni (Species C) An. aconitusAn. varunaAn. pampanaiNot identified Forest 60.91014.091.82 7.73 Agriculture 50.96013.8420.680.279.18 ALL 52.84013.8916.210.639.27 Percent of individuals identified morphologically as An. minimus

13. Conclusion Literature states deforestation may reduce the populations of An. dirus followed by invasion by other efficient vectors such as An. maculatus and An. minimus. Our data agrees with this and also suggests reestablishment of An. dirus in former forested areas. Species complex members demonstrate different behaviors, need to fully elucidate which members are present IN PROGRESS Correlate CSP+ data with species complex identification data IN PROGRESS

14. Future Study Observe one complete year (wet and dry seasons) of anopheline population bionomics. Determine which vectors are most efficient and how habitat change will drive infection Correlate mosquito infection rates with human malaria prevalence in the two sites Larval surveillance compare with adult bionomics information Susceptibility of species complex members to current methods of control (insecticide treated bed nets, pesticides)

15. Control of Adult Mosquito Vectors on Military Installations in Singapore Using Mass Trapping Techniques K. A. Barbara 1, A. Wilson 2, L. C. Ng 3, C. H. Tan 3, and G. T. Brice 1 1 US Naval Medical Research Unit #2 2 Navy Region Center Singapore 3 Singapore National Environment Agency

16. Navy Region Center Singapore Mass Trapping To determine the efficacy of a commercial mosquito trap (Mosquito Magnet Liberty Plus) in reducing prevalence of disease infected mosquito populations in a designated area Correlate trap catch with prevalence of infected mosquitoes and overall reduction in mosquito populations Determine the species composition and infection ratio of collected vectors

17. Navy Region Center Singapore Mass Trapping 2 weeks CDC light trap “pre-treatment” surveillance (baseline) Trap line of 4 Mosquito Magnets in three areas with CDC light traps in control and treatment areas 6 week “treatment” period 2 weeks “post-treatment” surveillance

18. Navy Region Center Singapore Mass Trapping Study initiated in January 2010 Currently collecting data First field trial scheduled to be completed in March Second field trial to be conducted on Singapore Armed Forces training grounds FY11 funding requested to continue study on islands off the coast of Cambodia and Indonesia

19. Future Studies NAMRU2 Entomology FY10 Febrile Syndrome Surveillance in Cambodia (vector component) FY10/FY11 Mass Trapping on islands off Cambodia and Palau Seribu area near Jakarta FY12 Field collections of vectors to be tested using rapid dipstick testing being developed at WRAIR

20. Acknowledgements The National Center for Parasitology, Entomology and Malaria Control, Phnom Penh, Cambodia National Environment Agency, Singapore Navy Region Center Singapore Public Works NAMRU2 entomology laboratory staff