1. A Modern Approach to Controlling ICP Spray Chamber Temperature Jerry Dulude, Glass Expansion, jdulude@geicp.com

2. Glass Expansion www.geicp.com 1 Limitations of Externally Controlled Spray Chambers Requires antifreeze solution Requires bulky floor model chiller Cumbersome coolant lines from chiller to chamber Freezing of condensate on lines Requires intricate jacketed chamber

3. Glass Expansion www.geicp.com 1 Limitations of other Peltier Controlled Spray Chambers Lack of programmability Insufficient cooling Require external fluid supply Limited temperature range Limited compatibility

4. Glass Expansion www.geicp.com 1 IsoMist™ Programmable Temperature Spray Chamber

5. Glass Expansion www.geicp.com 1 Features of IsoMist Powerful Peltier-effect chiller reaches -5C in 15 minutes Programmable from -10 to 60C in 1 degree increments Maintains temperature to within 0.1 degree Compact design (7.5x4x4 inches) 100% self-contained (no external lines) Incorporates Bluetooth® technology for clean wireless control (USB available)

6. Glass Expansion www.geicp.com 1 PC Screen showing IsoMist Software

7. Glass Expansion www.geicp.com 1 Variety of Cyclonic Spray Chambers Borosilicate glass: most aqueous and organic applications Quartz: Trace boron determinations PFA: HF and ultra trace applications

8. Glass Expansion www.geicp.com 1 Applications of the IsoMist Analyze volatile solvents – Low temp. Reduce oxide interferences – Low temp. Lower detection limits – High temp. Increase stability – Constant temp.

9. Glass Expansion www.geicp.com 1 Determination of Trace Metals in Naphtha Interfere with the cracking process Poisoning of the catalysts Environmental release concerns Origin and migration markers

10. Glass Expansion www.geicp.com 1 Spike Recoveries in Light Naphtha Direct (no dilution)

11. Glass Expansion www.geicp.com 1 Oxide Reduction in ICP-MS Isobaric oxide interferences Element/IsotopeInterference 56 Fe 40 Ar 16 O + 51 V +35 Cl 16 O + 44 Ca +14 N 14 N 16 O + 48 Ti +32 S 16 O + 52 Cr +34 S 18 O + 64 Zn +32 S 16 O 16 O + 64 Zn +48 Ca 16 O + Robert Thomas, Practical Guide to ICP-MS, Marcel Dekker, NY, 2004.

12. Glass Expansion www.geicp.com 1 Reducing Oxides for ICP-MS Data courtesy of David Jones, ALS Chemex, Brisbane, Australia

13. Glass Expansion www.geicp.com 1 Analysis of Limited Sample Volumes Biological applications Intracellular fluids Neonatal samples Forensic applications Requires micro flow nebulization Low DL’s desirable

14. Glass Expansion www.geicp.com 1 Effect of Temperature on Normalized LOD ( 20ul/min Uptake) PE Optima 2100DV

15. Glass Expansion www.geicp.com 1 Constant temperature benefits Environmental monitoring Tighter CCV’s Fewer QC’s Higher productivity Metal assays Tighter bracketing Higher accuracy

16. Glass Expansion www.geicp.com 1 IsoMist Customization PE ICP & ICP-MS Varian ICP & ICP-MS Agilent ICP-MS ThermoFisher ICP & ICP-MS Horiba J-Y ICP Teledyne Leeman ICP GBC ICP Spectro ICP Borosilicate glass Quartz Inert PFA Models Spray Chamber Materials

17. Glass Expansion www.geicp.com 1 Chassis Configurations of IsoMist

18. Glass Expansion www.geicp.com 1 IsoMist on Optima 2100DV

19. Glass Expansion www.geicp.com 1 IsoMist on Optima 2100DV

20. Glass Expansion www.geicp.com 1 IsoMist on the Agilent 7500 ICP-MS

21. Glass Expansion www.geicp.com 1 IsoMist on the Varian Vista MPX

22. Glass Expansion www.geicp.com 1 IsoMist on the Varian Vista MPX

23. Glass Expansion www.geicp.com 1 IsoMist on the Thermo iCAP 6500DV

24. Glass Expansion www.geicp.com 1 IsoMist on the PE Elan 9000

25. Glass Expansion www.geicp.com 1 IsoMist on the PE Elan 9000

26. Glass Expansion www.geicp.com 1 Summary Control of Spray chamber temperature enhances the capability of your ICP. The IsoMist is easily installed. The IsoMist is easily and precisely controlled. Compatible with all major ICP-OES & ICP-MS models.