1. Corbett Research CAS-1200 Dimensions Operates from PC or laptop
490 (W) x 580 (D) x 390 (H)
Operates from PC or laptop
Manufactured and supported in Brisbane

3. Pipetting Single pipetting head Pipette volumes as low as 1 µL
Repeatable to within 0.1 µL
50 µL and 200 µL tips
Filtered Conductive tips

4. Level Sensing Level sensing is standard
Requires conductive tips (black, carbon impregnated)
Limits tip immersion
More accurate pipetting

5. Software interface Intuitive layout Multiple options to suit user
Prepare master mix from reagents or add pre-prepared master mix
Dilute Standards each time or use pre-prepared dilutions
Normalize sample concentrations
Import/Export of sample names (from CSV or tab delineated file or barcode)
Wide range of plates can be used (384-, 96-, 72-, Gene-Disc etc)

6. Compatible with
1.5 ml tapered tubes
sample format
Compatible with Corbett X-tractor sample trays
Compatible with 0.2ml tubes

7. Rotor-Gene™ Gene-Disc™
Vertical tube orientation means a robot can set up all reactions directly into Gene-Disc tubes
The heat sealer provides a permanent or removable film seal (user selectable switch)
Gene-Disc rotor ready for cycling

8. Position and height
Before using the robot each block must have definitions for position then height
Once you have specified the position to the middle of well 1 on a selected plate all other wells are automatically calibrated
Height is defined either using either automatic or manual calibration
Recommend checking height automatically first then repeat manually, especially if using small volumes
If unsure do not change position and height as this will affect all users

9. Plate position
Select “Options” on the toolbar, then “Robot Setup , Calibrate plate positions
Choose plate then use X and y adjustments to select the middle of the first well

10. Plate height Select “Options”, “Robot Setup”, Calibrate plate heights”
Warning comes up to make sure you know what you are doing
Select plate, change 4 to 1, click autodetect
Repeat, change 4 to 1, and use manual Setup, lower tip until it feels tighter

11. Sample names and IDs can be imported from CSV or tab delineated files (also excel)

12. Starting the run
Click green arrow button on top toolbar

13. During the run

14. After the run

15. Error Messages

16. Tip usage options Robot can re-use tips when dispensing mastermix
Tip can be re-used 96 times if required
We recommend re-use 8-12 times
Tip re-use is less precise than single use tips
Tips can’t be re-used for samples going into a wet well
Robot programmed not to allow a tip that has been in a wet well (e.g. with MM) to be re-used
If pipetting sample into a dry well the same tip can be used

17. Jennifer McMahon 0414 278 369 www.corbettresearch.com
Phone:
FAX:
Freecall:

18. 20 µL reaction volume, 18 replicates
Rotor-Gene™ 3000 results
20 µL reaction volume, 18 replicates
Hand pipetting
CT std dev 0.07
CAS-1200 robot
CT std dev 0.05

19. 15 µL reaction volume, 18 replicates
Rotor-Gene™ 3000 results
15 µL reaction volume, 18 replicates
Hand pipetting
CT std dev 0.11
CAS-1200 robot
CT std dev 0.07

20. 10 µL reaction volume, 18 replicates
Rotor-Gene™ 3000 results
10 µL reaction volume, 18 replicates
Hand pipetting
CT std dev 0.12
CAS-1200 robot
CT std dev 0.10

21. 5 µL reaction volume, 18 replicates
Rotor-Gene™ 3000 results
5 µL reaction volume, 18 replicates
Hand pipetting
CT std dev 0.64
CAS-1200 robot
CT std dev 0.12