1. Multimistura is an alternative dietary supplement - high nutritional value - low cost - destined mainly to undernourished children - formulated with several ingredients - use of alternative ingredients - preference for locally available products

2. Multimistura May be formulated using different raw materials Flexibility to produce according to Regional availability Nutritional requirements of a given population

3. Multimistura Recent studies carried out at LRi/CENA/USP Composition assessment of different Formulations Constituents Findings Large variations among nutrient concentrations Possibility to produce different formulations Fulfill specific nutritional requirements

4. Multimistura nutritional value

5. Multimistura’s nutritional value is comparable to commercial supplements

6. Multimistura compared to commercial supplements nutritional composition

7. Multimistura compared to commercial supplements dietary minerals

8. Chemical elements in food usually present a large range of variation Multimistura and its ingredients should be carefully evaluated Selenium taken as example

9. Multimistura and its ingredients First results of chemical elements evaluated by instrumental neutron activation analysis (INAA)

10. Multimistura and its ingredients First results of chemical elements evaluated by instrumental neutron activation analysis (INAA)

11. Multimistura and its ingredients First results of chemical elements evaluated by instrumental neutron activation analysis (INAA)

12. Multimistura and its ingredients First results of chemical elements evaluated by instrumental neutron activation analysis (INAA)

13. Multimistura and its ingredients First results of chemical elements evaluated by instrumental neutron activation analysis (INAA)

14. Multimistura and its ingredients First results of chemical elements evaluated by instrumental neutron activation analysis (INAA)

15. Multimistura and its ingredients First results of chemical elements evaluated by instrumental neutron activation analysis (INAA)

16. Multimistura and its ingredients First results of chemical elements evaluated by instrumental neutron activation analysis (INAA)

17. Multimistura High potential for supplying mineral nutrients Extremely positive cost/benefit Example Recommended dose of a multimistura for 1-3 year old children = only 10 g/day Very low cost

18. Multimistura Example – mineral nutrients This small daily portion can supply the DRI requirements for All chromium and manganese 50% of magnesium Near one third of copper, iron and zinc

19. STUDY OF CHEMICAL ELEMENTS IN MULTIMISTURA AND ITS INGREDIENTS Why using neutron activation analysis?

20. Neutron activation analysis: - is a multi-element capable technique - does not need sample dissolution - can be performed at a high metrological level RELIABLE RESULTS!

21. Neutron activation analysis was recently approved by CCQM/BIPM as a primary method The President said that although the CCQM had not discussed the list of ‘potentially primary methods’ during recent years, it was recognised that NAA had claims to a similar status to that of the five methods listed originally by the CCQM and that NAA will be added to that list.

22. CCQM Primary Methods Isotope Dilution Mass Spectrometry Coulometry Gravimetry Titrimetry Determination of Freezing Point Depression Neutron Activation Analysis

23. Example of INAA performance compared to IDMS CCQM-K49/P85 – Bovine Liver Adapted from: R. R. Greenberg et al 2007 Preliminary Results RV +1 STD -1 STD CENA

24. Example of INAA performance compared to IDMS CCQM-P29/K24 – Rice Adapted from: Y. Aregbe et al 2003 Metrologia 40 KCRV +U -U

25. STUDY OF CHEMICAL ELEMENTS IN MULTIMISTURA AND ITS INGREDIENTS Why is CENA/USP qualified for such study?

26. Radioisotopes Laboratory – CENA/USP operates according to NBR ISO / IEC 17025 implementation started in1996 international recognition by IAEA in 2002 (IAEA: International Atomic Energy Agency) Quality System

27. CENA/USP hosts The Brazilian Satellite Centre of Trace Element Institute for UNESCO

29. Bureau International des Poids et Mésures (BIPM) Sèvres, Paris, France Consultative Committee for Amount of Substance (CCQM) Demonstration of analytical competence: Metrology in Chemistry – participation in CCQM

30. Participation in CCQM – Inorganic Analysis Working Group - 14 participations - 12 elements - 12 materials Metrology in Chemistry

31. SEVERAL PARTICIPATIONS IN THE CCQM INVOLVING FOOD SAMPLES SOME EXAMPLES Metrology in Chemistry

32. CCQM-P39.1 and CCQM-K43 – Salmon Adapted from: Y. Aregbe et al 2006 Metrologia 43 KCRV +U -U

33. CCQM-P29/K24 – Rice Adapted from: Y. Aregbe et al 2003 Metrologia 40 KCRV +U -U

34. CCQM-K49/P85 – Bovine Liver Adapted from: R. R. Greenberg et al 2007 Preliminary Results RV +1 STD -1 STD CENA

35. CCQM-P39 – Tuna Fish Adapted from: Y. Aregbe et al 2004 Metrologia 41 Reference Value CI 95%

36. CCQM-P64 – Nonfat Soybean Powder Adapted from: Ma Liandi et al 2006 Report – Draft A RV +2 STD -2 STD CENA

37. Welcome to the Radioisotopes Laboratory… Waterpaint by Prof. Klaus Reichardt ( Nikolaus ), 1996 Stained-glass door of the gamma spectrometry room