2. Pet Allergen Diagnosis Protein Array Excelsior Bio-system Kang Hsin Chen Excelsior Bio-system Kang Hsin Chen

3. What is Allergic Reaction? Over reaction to common environment substance –Mite 、 pollen 、 hair 、 fungi –Soybean 、 wheat 、 milk 、 pork etal. Over reaction to common environment substance –Mite 、 pollen 、 hair 、 fungi –Soybean 、 wheat 、 milk 、 pork etal.

4. What Causes for Allergies? Type I acute anaphylactic reaction –IgE - degranulation of Mast cell –Mediator released –Atopy 、 Dermatitis 、 Asthma Type I acute anaphylactic reaction –IgE - degranulation of Mast cell –Mediator released –Atopy 、 Dermatitis 、 Asthma

5. IgE - Degranulation of Mast Cells Cell-bound form IgE

6. Mediator Released

7. Atopic Canine Atopic Dermatitis (AD) –inhalent or GI-intake? Age of the onset: 1-3 years Clinical presentation: pruritus 、 erythema 、 chewing 、 licking 、 salivary staining Atopic Dermatitis (AD) –inhalent or GI-intake? Age of the onset: 1-3 years Clinical presentation: pruritus 、 erythema 、 chewing 、 licking 、 salivary staining

8. Clinical presentation

9. Health Care of Atopic Pet Rule out other disease Avoid second infection Pet’s serum specific IgE profile Food design based on allergen result immunotherapy Rule out other disease Avoid second infection Pet’s serum specific IgE profile Food design based on allergen result immunotherapy

10. Traditional Diagnosis Clinical signs and history Skin test Serological test –ELISA/ –RAST(radioallergosorbent test) Clinical signs and history Skin test Serological test –ELISA/ –RAST(radioallergosorbent test)

11. What defect about skin test? Shaved the hair Dangerous to pet Food allergen unable 8-10 allergen could be tested at one time Shaved the hair Dangerous to pet Food allergen unable 8-10 allergen could be tested at one time

12. What defect about traditional serological test? Too many false negative results –Sensitivity is not enough Too many false positive results –Specificity is not enough Score is confused – Signal range is too small Too many false negative results –Sensitivity is not enough Too many false positive results –Specificity is not enough Score is confused – Signal range is too small

13. Pet Allergen Panel in Taiwan

14. Our Total Solution Immunostrip Allergen Array Health Food –Probiotic Balance TH1/TH2 –Low allergic Food Formula based on test result Immunostrip Allergen Array Health Food –Probiotic Balance TH1/TH2 –Low allergic Food Formula based on test result

15. Immunostrip Rule out other possibility First line screening Fast and Easy Rule out other possibility First line screening Fast and Easy

16. Immunostrip

17. Technology- Allergen Preparation More than 200 items of allergens purified –Covering a variety range of food and air-borne allergens, including dust mites, animal hairs, pollens, Chinese herbals, meat and vegetables etc. –Whole proteins extracted by customized rapid buffer extraction and size exclusion processes according to different types of raw material Quality control and assurance established –SDS-PAGE analyzed for batch to batch control –Major allergen items were tested and confirmed by clinical samples (both human and canine) More than 200 items of allergens purified –Covering a variety range of food and air-borne allergens, including dust mites, animal hairs, pollens, Chinese herbals, meat and vegetables etc. –Whole proteins extracted by customized rapid buffer extraction and size exclusion processes according to different types of raw material Quality control and assurance established –SDS-PAGE analyzed for batch to batch control –Major allergen items were tested and confirmed by clinical samples (both human and canine)

18. mitepollenfungiFood-1Food-2

19. Fluorescence Allergen Array Include 62 allergic items Design for Taiwan area Provide the specific IgE profile Include 62 allergic items Design for Taiwan area Provide the specific IgE profile

20. Principle of Allergic Array Array Spotter

21. Principle of Allergic Array

22. Laser Scanner

23. Example of Allergen Array Results Hypersensitivity Hyposensitivity

24. Our 62 Panel Set Mite –Blomia tropicals stoirge mite –Dermatophagoides pteronyssinus –Dermatophagoides farinae –Acarus Siro –Tyrophagus Putrescentiae Mite –Blomia tropicals stoirge mite –Dermatophagoides pteronyssinus –Dermatophagoides farinae –Acarus Siro –Tyrophagus Putrescentiae

25. Our 62 Panel Set Insect and others –Tsutsugamushi mite –Tick –Flea –Aedes Communis –Cules Pipiens –Blatella Germanica Insect and others –Tsutsugamushi mite –Tick –Flea –Aedes Communis –Cules Pipiens –Blatella Germanica

26. Our 62 Panel Set Dander and Skin scrape –Columba Palumbus –Human Dander –Cow hair and skin scrape –Sheep hair and skin scrape –Cat hair Dander and Skin scrape –Columba Palumbus –Human Dander –Cow hair and skin scrape –Sheep hair and skin scrape –Cat hair

27. Our 62 Panel Set Fungus –Cladosporium Herbharum –Candida albicans –Alternaria alternate –Penicillium notatum –Aspergillus fumigatus Fungus –Cladosporium Herbharum –Candida albicans –Alternaria alternate –Penicillium notatum –Aspergillus fumigatus

28. Our 62 Panel Set Pollen –Eucalyptus globules –Poa Pratensis –Amaranthus retroflexus –Rumex Acetosella –Ambrosia Artemisii folia(elatior) –Acacia Baileyana –Cynodon Dactylon Pollen –Eucalyptus globules –Poa Pratensis –Amaranthus retroflexus –Rumex Acetosella –Ambrosia Artemisii folia(elatior) –Acacia Baileyana –Cynodon Dactylon

29. Our 62 Panel Set Foods –Milk 、 egg white 、 egg yolk 、 cheese –Duck 、 lamb 、 beef 、 chicken 、 turkey 、 pork –Soybean 、 peanut 、 rice 、 wheat 、 carrot 、 corn 、 patatoe –crab 、 shrimp 、 mackerel 、 sardine 、 anchovy 、 cod fish 、 salmon –watermelon 、 mango 、 cantaloupe 、 pear 、 mandarine 、 banana 、 apple –Yeast baker's 、 yeast brewer's Other –Flax Foods –Milk 、 egg white 、 egg yolk 、 cheese –Duck 、 lamb 、 beef 、 chicken 、 turkey 、 pork –Soybean 、 peanut 、 rice 、 wheat 、 carrot 、 corn 、 patatoe –crab 、 shrimp 、 mackerel 、 sardine 、 anchovy 、 cod fish 、 salmon –watermelon 、 mango 、 cantaloupe 、 pear 、 mandarine 、 banana 、 apple –Yeast baker's 、 yeast brewer's Other –Flax

30. Our Results From 2007/1-2007/6 183 Pets (canine) 81% canine have allergic reaction 4 different score –No reaction/minor/medium/serious From 2007/1-2007/6 183 Pets (canine) 81% canine have allergic reaction 4 different score –No reaction/minor/medium/serious

31. Top 10 allergens Blomia tropicals stoirge mite39% Tyrophagus Putrescentiae35% Amaranthus retroflexus29% Aspergillus fumigatus27% Alternaria alternate27% Dermatophagoides pteronyssinus26% Ambrosia Artemisii folia(elatior)22% Cynodon Dactylon21% Dermatophagoides farinae19% Acarus Siro19% Yeast brewer's31% Corn22% Milk19% Egg white18% Yeast baker's15% Cheese13% Egg yolk13% cod fish13% Lamb11% Wheat11% InhalantGI-intake

32. SummarySummary Benefits of array based allergens test

33. No more need for sedation No more need for shave the pets No more need a lot of sera Item is flexible for different area or customer need No more need for sedation No more need for shave the pets No more need a lot of sera Item is flexible for different area or customer need

34. Benefits of array based allergens test We can test food allergens We can economize your time and money You can get pet IgE profile at one test You can list of foods that the pet should avoid We can test food allergens We can economize your time and money You can get pet IgE profile at one test You can list of foods that the pet should avoid

35. Thank You!