1. H OST - RANGE STUDY ON THE STEM - BORING WEEVIL (L ISTRONOTUS SETOSIPENNIS (C OLEOPTERA : C URCULIONIDAE ) FOR THE CONTROL OF P ARTHENIUM HYSTEROPHORUS IN E THIOPIA By Sintu Alemayhu

2. O BJECTIVE To evaluate the safety of the stem-boring weevil, Listronotus setosipennis non-target plant species

3. D ESCRIPTION AND LIFE CYCLE stem boring weevil Listronotus setosipennis (Hustache) [Coleoptera: Curculionidae] originally from Argentina, were introduced from Agricultural Research Council-Plant Protection Research Institute (ARC-PPRI) of South Africa in December 2009, to be used for studies in Ethiopia

4. Nocturnal adults feed on leaves and flowers Lay eggs in flower heads or leaf bases hatch 3-5 days later pupate in soil inside fibrous capsules Listronotus setosipennis (Coleoptera: Curculionidae Complete life cycle of Listronotus takes 30-35 days.

5. DAMAGE o Adult feeding damage is negligible. o Larval feeding has the ability to kill or prevent further development of parthenium seedlings. In glasshouse conditions, infestation by Listronotus weevil at high densities significantly reduced the plant biomass by 21.9% and flower production by 75%.

8. H OST SPECIFICITY o Host specificity testing is a critically important step in the process of introducing natural enemies for classical weed biological control. o It provides information upon which the suitability of a proposed biological control agent can be assessed (Marohasy1998).

9. C ONT … Evaluation of the host range of any bioagent comprises of no-choice tests (test plant species only), followed by choice tests (test plant with Parthenium) if feeding, oviposition or development is observed under no-choice testing on non-target species The safety of Listronotus setosipennis (Hustache) to non-target plants was tested under quarantine on economically important members of the Asteraceae family and indigenous weed species through no-choice tests

10. S ELECTION OF NON - TARGET SPECIES The selection of non-target species for host preference testing was made by a taxonomist from Addis Ababa University. The centrifugal phylogenetic method was followed in selecting test plants for testing candidate control agents The test plant species were identified based on how closely related they are to Parthenium, whether they are strictly endemic, near endemic, cultivated, or indigenous to Ethiopia.

11. Figure 1. Diagrammatic representation of the tribe Heliantheae and some crop plants (adapted by Lorraine Strathie from Wapshere, A. J. 1974).

12. M ATERIALS AND M ETHODS Area description The experiment was conducted under quarantine condition at Ambo Plant Protection Research Center (APPRC) 109km West of Addis Ababa. The average temperature is ranges from 10 o to 30 o C and relative humidity at 50-87 % in the quarantine facility

13. C ULTURING OF L. SETOSIPENNIS Adult feeding and oviposition were tested using nylon mesh test cages with metal frames(0.5m x 0.5m x 1m), with two holes for potted plants. All corners and joints were sealed with silicone to prevent escape of weevils through gaps.

14. The safety of Listronotus setosipennis (Hustache) to 39 non-target plants consisting of nineteen major crop species mostly used as food and export Twenty crops and other plant species, belonging to the family Asteraceae which includes two species of Helianthus annus, six species Guizotia abyssinica, both of which are important oil crops in the country. and four species of Bidens

15. C ONT … o No-choice tests were designed to test whether the bio-agent feeds, lay eggs and survive on a test plant when it has no access to target weed Parthenium. o All the test plants were grown or transplanted into plastic pots(22cm X 18 cm), containing the mixture of sand 25%, compost/humus 25% and 50% soil o grown up to the required test stage by providing every agronomic management under lath-house

19. C ONT … o Healthy and similar size Plant with flowers, were selected and placed in a cage after being washed o Five mating pairs of adult L. setosipennis, obtained from same age group of adults that had been kept for 2-3 weeks, were released into each cage. o Sodium hypochlorite solution (2%) was sprayed on the leaves and soil to prevent any disease occurrence. o The experiment was replicated three times in CRD and one plant per cage was used.

21. C ONT … After 7 days of exposure all adults were removed from each test plant and the number of adults that survived, died and missed was recorded All plant parts, including leaf axils, stems and petioles, were carefully examined for ovipostion and feeding. Eggs were counted after dissecting flowers and examining plants under a microscope.

22. The blue arrow shows frass The read arrow shows egg

23. Tagetes minutaHelianthus annus (oissa) Bidens pachyloma G.abysinica (kuyyu) Vernonia galamensis

24. NoScientific NameCommon name / variety Total no of egg Total meanS.DS.E Parthenium hystrerophorous (control) partehnium864838.9650.013.37 1. Eragrostis tefTeff 000.00 2. Eragrostis tefTeff v DZ-c37 000.00 3. Sorghum bicolorSorghum 000.00 4. Zea maysMaize 000.00 5. Hordeum vulgareBarley 000.00 6. Triticum aestivumWheat 000.00 7. Cicer arietinumChick pea 000.00 8. Lens cultinarisLentil 000.00 9. Gossipium sp.Cotton var, Arba 000.00 10. Sesamum indicum Sesame 000.00 var. Abasena 11. Sesamum indicum var. Tate 000.00 12. Sesamum indicum var.5 000.00 13. Pisum sativum L.Field peas 000.00 14. Vicia fabaFaba beans 000.00 15. Lathyrus sativusGrass pea, Indian vetch 000.00 16. Solanum tuberosumPotato local 000.00 17. Solanum lycopersicumTomato 000.00 18. Capsicum sp.Pepper 000.00 19 Corchorus sp. 000.00 Table 1. Result of Host-Specificity of Listronotus setosipennis (Hustache) on major crops

25. Evaluation of species related to Parthenium NoScientific/variety nameCommon nameTotal no of egg Total meanS.DS.E 1.Guizotia abyssinica (Local)niger seed0 00.00 2.G. abyssinica (Fogera)niger seed0 00.00 3.G. abyssinica (ESTE)niger seed0 00.00 4.G. abyssinica (Kuyu)niger seed0 00.00 5.G. abyssinica (Shambu)niger seed0 00.00 6.G. abyssinica (Ghinchi)niger seed0 00.00 7.Helianthus annuus (oissa)sunflower0 00.00 8.Helianthus annuus (R.B)sunflower0 00.00 9.Carthamus tinctorius Safflower 0 00.00 10. Vernonia galamensisIron Weed 0 00.00 11. Lactuca sativaLettuce 0 00.00 12. Bidens pilosaBlack jack 0 00.00 13 Bidens PachylomaAdeyabeba (Amharic) 0 00.00 14 Bidens ghedoensis 0 00.00 15 Bidens sp 0 00.00 16 Guizotia scabraMetch (Amharic) 0 00.00 17. Conyza bonariensisHorseweed, Asthmaweed 0 00.00 18. Galinsoga parvifloraGallant soldier 0 00.00 19. Tagetes minutaBlack mint 0 00.00 20. Flaveria trinervia Clustered yellow tops0 00.00 21.Parthenium hysterophorusparthenium8648 38.9650.013.37

26. C ONCLUSION The concern that the agent, Listronotus setosipennis, might attack non- target crops and plants was resolved through a quarantine test against crops and plants that are closely related to Parthenium and others that are economically important and indigenous to Ethiopia. Therefore there was no need to go for choice test since no oviposition took place in no choice test