1. Kitten with Pruritis Dennis B. DeNicola, DVM, PhD, DACVP Chief Veterinary Educator IDEXX Laboratories

2. “Kitten” – 6 month old DSH 6 months old Intact female Domestic Shorthair Presented with clinical history of pruritis Cat was depressed upon presentation but became extremely excited during blood collection process

3. “Kitten” – 6 month old DSH WBC=17.76 K/  L(5.50 – 19.50) LYM=2.36 K/  L(0.90 – 7.00) MONO=1.78 K/  LHIGH(0.10 – 0.79) NEU=12.29 K/  L(2.50 – 12.50) EOS=1.29 K/  L(0.10 – 0.79) BASO=0.05 K/  L(0.00 – 0.10) %LYM=13.3% %MONO=10.0% %NEU=69.2% %EOS=7.2% %BASO=0.3% HCT=23.9%LOW(30.0– 45.0) RBC=6.11M/  L( 5.00 – 10.00 ) HGB=8.5g/dL( 8.0 – 15.1 ) RETIC=102.8K/  L %RETIC=1.7% MCV=39.1fLLOW ( 41.0 – 58.0 ) RDW=22.7%HIGH( 17.3 – 22.0) MCHC=35.5g/dL( 29.0 – 36.0 ) MCH=13.86pg(12.50 – 17.60) PLT=905 K/  LHIGH (175 – 600) MPV=16.69fL PCT=1.5% PDW=17.9%

4. “Kitten” – 6 month old DSH Erythron HCT=23.9%LOW( 30.0 – 45.0 ) RBC=6.11M/  L( 5.00 – 10.00 ) HGB=8.5g/dL( 8.0 – 15.1 ) RETIC=102.8K/  L %RETIC=1.7% MCV=39.1fLLOW( 41.0 – 58.0 ) RDW=22.7%HIGH( 17.3 – 22.0) MCHC=35.5g/dL( 29.0 – 36.0 ) MCH=13.86pg(12.50 – 17.60)

5. “Kitten” – 6 month old DSH Erythron HCT=23.9%LOW( 30.0 – 45.0 ) RBC=6.11M/  L( 5.00 – 10.00 ) HGB=8.5g/dL( 8.0 – 15.1 )

6. “Kitten” – 6 month old DSH Erythron HCT=23.9%LOW( 30.0 – 45.0 ) RBC=6.11M/  L( 5.00 – 10.00 ) HGB=8.5g/dL( 8.0 – 15.1 ) Anemia Mild

7. “Kitten” – 6 month old DSH Erythron HCT=23.9%LOW( 30.0 – 45.0 ) RBC=6.11M/  L( 5.00 – 10.00 ) HGB=8.5g/dL( 8.0 – 15.1 ) RETIC=102.8K/  L %RETIC=1.7%

8. “Kitten” – 6 month old DSH Erythron HCT=23.9%LOW( 30.0 – 45.0 ) RBC=6.11M/  L( 5.00 – 10.00 ) HGB=8.5g/dL( 8.0 – 15.1 ) RETIC=102.8K/  L %RETIC=1.7% Anemia Mild, regenerative

9. “Kitten” – 6 month old DSH Erythron HCT=23.9%LOW( 30.0 – 45.0 ) RBC=6.11M/  L( 5.00 – 10.00 ) HGB=8.5g/dL( 8.0 – 15.1 ) RETIC=102.8K/  L %RETIC=1.7% MCV=39.1fLLOW( 41.0 – 58.0 ) RDW=22.7%HIGH( 17.3 – 22.0) MCHC=35.5g/dL( 29.0 – 36.0 ) MCH=13.86pg(12.50 – 17.60)

10. “Kitten” – 6 month old DSH Erythron HCT=23.9%LOW( 30.0 – 45.0 ) RBC=6.11M/  L( 5.00 – 10.00 ) HGB=8.5g/dL( 8.0 – 15.1 ) RETIC=102.8K/  L %RETIC=1.7% MCV=39.1fLLOW( 41.0 – 58.0 ) RDW=22.7%HIGH( 17.3 – 22.0) MCHC=35.5g/dL( 29.0 – 36.0 ) MCH=13.86pg(12.50 – 17.60) Anemia Mild, regenerative, microcytic and potential mild anisocytosis

11. Erythron – Data Interpretation Anemia Mild Microcytic (decreased MCV) Regenerative (increased Reticulocyte Count) Potential mild anisocytosis (increased RDW)

12. Erythron – Blood Film

18. Erythron – Blood Film Interpretation Anemia Mild Regenerative - reticulocytosis Polychromasia Anisocytosis

19. Erythron – Blood Film Interpretation Anemia Mild Regenerative - reticulocytosis Polychromasia Anisocytosis Blood loss anemia Developing Iron deficiency

20. “Kitten” – 6 month old DSH Leukon WBC=17.76K/  L( 5.50 – 19.50 ) LYM=2.36K/  L( 0.90 – 7.00 ) MONO=1.78K/  LHIGH( 0.10 – 0.79 ) NEU=12.29K/  L( 2.50 – 12.50) EOS=1.29K/  L( 0.10 – 0..79 ) BASO=0.05K/  L( 0.00 – 0.10 ) %LYM=13.3% %MONO=10.0% %NEU=69.2% %EOS=7.2% %BASO=0.3%

21. “Kitten” – 6 month old DSH Leukon WBC=17.76K/  L( 5.50 – 19.50 ) High normal leukocyte count

22. “Kitten” – 6 month old DSH Leukon WBC=17.76K/  L( 5.50 – 19.50 ) LYM=2.36K/  L( 0.90 – 7.00 ) MONO=1.78K/  LHIGH( 0.10 – 0.79 ) NEU=12.29K/  L( 2.50 – 12.50) EOS=1.29K/  L( 0.10 – 0..79 ) BASO=0.05K/  L( 0.00 – 0.10 ) %LYM=13.3% %MONO=10.0% %NEU=69.2% %EOS=7.2% %BASO=0.3%

23. “Kitten” – 6 month old DSH Leukon WBC=17.76K/  L( 5.50 – 19.50 ) LYM=2.36K/  L( 0.90 – 7.00 ) MONO=1.78K/  LHIGH( 0.10 – 0.79 ) NEU=12.29K/  L( 2.50 – 12.50) EOS=1.29K/  L( 0.10 – 0..79 ) BASO=0.05K/  L( 0.00 – 0.10 ) %LYM=13.3% %MONO=10.0% %NEU=69.2% %EOS=7.2% %BASO=0.3% High normal leukocyte count Mild monocytosis

24. “Kitten” – 6 month old DSH Leukon WBC=17.76K/  L( 5.50 – 19.50 ) LYM=2.36K/  L( 0.90 – 7.00 ) MONO=1.78K/  LHIGH( 0.10 – 0.79 ) NEU=12.29K/  L( 2.50 – 12.50) EOS=1.29K/  L( 0.10 – 0..79 ) BASO=0.05K/  L( 0.00 – 0.10 ) %LYM=13.3% %MONO=10.0% %NEU=69.2% %EOS=7.2% %BASO=0.3% High normal leukocyte count Mild monocytosis Low normal lymphocytes in face of excitement !

25. “Kitten” – 6 month old DSH Leukon WBC=17.76K/  L( 5.50 – 19.50 ) LYM=2.36K/  L( 0.90 – 7.00 ) MONO=1.78K/  LHIGH( 0.10 – 0.79 ) NEU=12.29K/  L( 2.50 – 12.50) EOS=1.29K/  L( 0.10 – 0..79 ) BASO=0.05K/  L( 0.00 – 0.10 ) %LYM=13.3% %MONO=10.0% %NEU=69.2% %EOS=7.2% %BASO=0.3% High normal leukocyte count Mild monocytosis Low normal lymphocytes in face of excitement ! High normal eosinophils with possible “stress”

26. Erythron – Blood Film

35. Leukogram Interpretation Normal leukocyte count No lymphocytosis in face of excitement Probable underlying glucocorticoid influence (“stress”) High normal eosinophil count in face of possible glucocorticoid influence (“stress”) High probability of underlying eosinophilic inflammatory condition

36. “Kitten” – 6 month old DSH WBC=17.76 K/  L(5.50 – 19.50) LYM=2.36 K/  L(0.90 – 7.00) MONO=1.78 K/  LHIGH(0.10 – 0.79) NEU=12.29 K/  L(2.50 – 12.50) EOS=1.29 K/  L(0.10 – 0.79) BASO=0.05 K/  L(0.00 – 0.10) %LYM=13.3% %MONO=10.0% %NEU=69.2% %EOS=7.2% %BASO=0.3% HCT=23.9%LOW(30.0– 45.0) RBC=6.11M/  L( 5.00 – 10.00 ) HGB=8.5g/dL( 8.0 – 15.1 ) RETIC=102.8K/  L %RETIC=1.7% MCV=39.1fLLOW ( 41.0 – 58.0 ) RDW=22.7%HIGH( 17.3 – 22.0) MCHC=35.5g/dL( 29.0 – 36.0 ) MCH=13.86pg(12.50 – 17.60) PLT=905 K/  LHIGH (175 – 600) MPV=16.69fL PCT=1.5% PDW=17.9%