1. CDC/NIH Vertebrate Animal Biosafety Level Criteria ► For all Animal Biosafety Levels (1 - 4)  IACUC Approval  Authorized access to facilities  Animal handling training  Medical surveillance program  Written safety manual(s)  Pest control program

2. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► For all Animal Biosafety Levels (1 - 4)  no eating, drinking, smoking, touching face  no food or drink storage within facilities  minimize aerosols  decontaminate work surfaces, equipment  transport wastes in leak-proof covered containers  handle sharps safely

3. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► For all Animal Biosafety Levels (1 - 4)  Protective clothing recommended, not worn outside facility  Facilities separate from general building traffic  External doors self-closing/self-locking  Animal room doors open inward, self-closing  Water resistant, easily cleaned surfaces (horizontal surfaces kept to a minimum)

4. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► For all Animal Biosafety Levels (1 - 4)  Sealed, break resistant windows  Floor drain traps filled with water or disinfectant  No recirculation of exhaust air  Rooms have negative pressure gradient to adjacent hallway  Hand washing sink available in facility  180 F cage wash rinse temperature  Adequate illumination

5. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 2 (ABSL2)  Restrict access to few as possible (advise support staff of potential hazards)  Biohazard sign posted on animal room entrance (hazard ID, contact info., & entry requirements)  Immunizations, serum surveillance  Labeled leakproof carriers, exterior disinfected before transport (wastes, tissues, etc.)  Safe sharps policies (plasticware, safe sharps devices, limited use of needles/syringes)

6. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 2 (ABSL2)  Limited to animals dedicated to work  Exposures, spills reported immediately  Gloves, gowns, uniforms or lab coats worn in rooms (removed prior to exit)  Face protection (goggles, safety glasses, full face shields, masks) selected on basis of risk  Biosafety cabinets used to confine aerosol procedures

7. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 2 (ABSL2)  Filter top cages, cage dumping containment stations used where appropriate  Autoclave available  hand washing sink in animal room

8. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 3 (ABSL3)  Individuals at increased risk of infection not allowed in facility  All wastes treated before disposal as regulated biomedical waste (incineration recommended)  Specialized training provided and updated  Cages autoclaved/decontaminated before bedding is removed  Spill procedures posted

9. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 3 (ABSL3)  Uniforms or scrub suits covered by solid-front gown  Face/eye, and where necessary, respiratory protection worn by all who enter animal rooms  Boots, shoe covers, etc. worn when needed  Consider use of containment ventilated cage units, housing in biosafety cabinets, of filter bonnets.

10. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 3 (ABSL3)  All procedures performed in biosafety cabinet (wear respiratory protection if working outside cabinet)  Consider clothes change room and shower  Penetrations in rooms are sealed or capable of being sealed  Hands-free sink near exit door  Sink-trap filled with disinfectant after each use

11. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 3 (ABSL3)  Ducted exhaust ventilation system  Exhaust discharge away from air intakes,  Consider HEPA filtration of exhaust air  Room airflow monitoring device (personnel verify airflow direction upon entry)  Consider alarms for ventilation system failure  Consider interlock of exhaust/supply systems to avoid pressurization

12. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 3 (ABSL3)  Biosafety cabinets tested/certified annually  Autoclave located near BL3 suite  Vacuum system protected with HEPA filter if used  Initial and annual verification of BL3 facility design parameters  Consider additional facility enhancements as needed (effluent decontamination, showers, etc)

13. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 4 (ABSL4)  Program includes post-exposure counseling and prophylaxis  Site-specific biosafety manual prepared  Double-door pass through autoclave  Procedures performed in pairs  Materials unrelated to work not allowed in facility  Additional access control measures (security guard)

14. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 4 (ABSL4)  Complete clothes change, personal shower upon exit  All material in/out of facility through pass through autoclave or disinfectant chamber  Autoclave door interlocked to prevent both open simultaneously  Chart exposures, accidents/incidents, employee absenteeism, appearance of related signs/symptoms of infection

15. CDC/NIH Verterbrate Animal Biosafety Level Criteria ► Animal Biosafety Level 4 (ABSL4)  Serum samples analyzed at defined intervals  Positive pressure suits or Class III biosafety cabinet line  Very unique engineering requirements for BL4 laboratories (dedicated building within building concept)