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Published byDiana Ken Modified over 9 years ago
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Effect of High CO 2 on Photosynthesis and Growth in Marine Phytoplankton Daniel Steurer Morel Lab, Summer 2011
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Trichodesmium Oceanic, non-heterocystous cyanobacteria Varies from other diazotrophs Major player in oceanic nitrogen fixation Primarily located in tropical and sub-tropical oceans Diurnal, following circadian rhythm in nitrogen fixation
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The Issue Fe’ in YBC II = ~200nM Fe’ in GSW = ~1250pM
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Varying EDTA
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Conclusions Varying EDTA levels has a large effect on growth rate of Trichodesmium in YBC II medium EDTA chelates potentially toxic ions in the relatively ‘dirty’ medium – e.g. copper YBC II obviously has some previously unnoticed detriments to growth Other potential unknown effects of the medium could exist
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Kranz, 2010 Shown: higher pCO 2 yields higher N 2 fixation rate in both instances *Grown in YBC II
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Shi and Morel, in prep. Shown: higher pCO 2 yields lower N 2 fixation rate in both instances *Grown in GSW
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Results / Analysis Nanomoles Fe/ug Chlorophyll a 0.41684586 0.44022874 0.31428607 0.41893009 MeanStandard Deviation 0.3980.057 YBC II, Fe’ = ~200nM GSW, Fe’ = ~1250 pM Nanomoles Fe/ug Chlorophyll a Mean Standard Deviation 3.0670.377 Clearly, the iron imbalance outside of the cells is not mimicked within the cells.
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Thanks to… Dalin Shi Francois Morel and co. Princeton Environmental Institute
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