1. Erectile Function Feeding Behavior--Obesity Pigmentation--Tanning Reproductive Ability Cardiovascular Function Learning Memory Immune Response HOT HOT Functions of  -MSH Analogues

2. Brief History of  -MSH Analogues

3. Synthesis of WY012 by SPPS

4. UGLY Facts related to Boc-(1-Me)His COST: US$645/g Synthesis Hydrophilicity assessed by HPLC (1’-Me)His and Boc-(1’-Me)His (Column dead volume is around 3.2 minutes)

5. Proposed Tactics--Using DIEA Salts

6. Reality of the Synthesis of DIEA Salts

7. HPLC Analysis of Crude Peptides Column: Vydac C 18 ; gradient: 10-90% A in 40 minutes [A: MeCN, B: (0.1% TFA) H 2 O]; flow: 1.0 mL; detection: 230, 254, 280 nm. a) From Boc-(1’-Me)His-OH; b) and c) From Boc-(1’-Me)-His DIEA and DIEA.HCl salts mix.

8. ConclusionsConclusions Mixture of Boc-(1’-Me)His.DIEA and DIEA.HCl salts was Successfully Applied to Peptide Syntheses This Method has Various Advantages, Including: Very Economic, Time-saving and Easy Access to Starting Materials This Method Should be Applicable to Other Hydrophilic Amino Acids

9. References and Acknowledgements 1. Eifler, S., Leblond, I., Trifilieff, E., and Lepoittevin, J.- P., Letters Pept. Sci., 4(1997) 467. 2. Pennington, M.W., and Dunn, B.M., (Eds.), Peptide Synthesis Protocols, Humana Press Inc., 1994. 3. Miyazawa, T., Otomatsu, T.; Yamada, T.; Kuwata, S., Chem. Express, 4(1989) 745. US Public Health Service Funded by Grants From US Public Health Service Reprint: http://www.chem.arizona.edu/faculty/hrub/hruby.html Contact: ghan@u.arizona.edu, hruby@u.arizona.edu