1. 1 PCR RESULTS! Josef Lautin Erica Forsberg Zdravka Boskovic Henrik Kleinhans Adam Kundurakis Håkan Lundberg

2. 2 First PCR run 25 cycles (30 sec + 30 sec + 1 min ) + 5 min 95 °C + 65 °C + 72 °C Polymerase: Taq Results: None

3. 3 Results of first PCR run Our vector (red) and lack of PCR product (orange)

4. 4 Second PCR run 25 cycles 1 min + (15 sec + 15 sec + 15 sec) 95 °C + 60 °C + 72 °C Polymerase: DreamTaq Results: Good

5. 5 Results of second PCR run Our second PCR run result

6. 6 Results of third PCR run

7. 7 Results discussion First 3 wells (red) were from our own colonies, 4 th one (orange) from Lasse. 3 rd one, not the expected DNA-fragment.

8. 8 What might have gone wrong First PCR might have been a pipetation error. Third PCR, third colony: The PCR prep. might have failed, since if there was a colony it should have been ok.