1. SMASH 2008 Recap: Introducing Citation, Your Hard- Working Lab Assistant Dean Olson, Ph.D. Director of Customer Support Protasis Corporation

4. Citation: Push-Button Sample Loader Chromatography-grade Fluidics Easy-to-use, Web-based Software Built-in Instrument Controller Integral Degassing Field-serviceable Valves Small-footprint benchtop packaging Enjoy the reassurance of feedback control, auto-calibration, and system self-maintenance in applications where the robot is not required Completely upgradeable to full robotic automation

5. Chromatography grade pumps Field-serviceable valves Integrated feedback control sensors Degas, blanket, sparge On-board instrument controller Protasis One-Minute™ NMR Lite Software

7. The size of a plant metabolome is unknown it is estimated at a few thousand constituents Plant metabolomics LC-NMR 10 -6 mol LC-UV10 -9 mol GC-MS 10 -12 mol LC-MS 10 -15 mol LC-LIF10 -19 mol CE-LIF 10 -22 mol Sumner et al. Phytochemistry 2003, 62, 817-836. Rapid, Reproducible and Non-Destructive Low Sensitivity NMR Metabolomics High Sensitivity Instrumentation variability LC-MS: problem in RT repeatability MS Metabolomics

8. CONTROL WOUNDED PCA of total crude extract MS spectra spiked Total crude extract TOF MS spectra RANKED LIST OF INDUCED IONS Total crude extract TOF MS spectra CONTROL WOUNDED C 12 H 17 O 3 209.1256 Data Courtesty of Jean-Luc Wolfender, University of Geneva, Switzerland

9. UPLC-TOF-ES-MS of A. thaliana Induced by wounding m/z 209.12 (+/- 10 ppm) m/z 209.12 (+/- 0.5 Da) CAP-NMR Multiple collection triggered by MS Data Courtesty of Jean-Luc Wolfender, University of Geneva, Switzerland

10. CAP-NMR of ion m/z 209 CAP-NMR ca 1  g NT=5000 12 10973,2,811,5412 Standard 50  g 11 8 7 5 4 3 2 1 9 10 Data Courtesty of Jean-Luc Wolfender, University of Geneva, Switzerland

11. Further example details can be found in… A LC-MS approach is presented for the isolation of minor key plant biomarkers, in view of their characterization by NMR at the microgram scale …using ultra-performance liquid chromatography- time-of-flight mass spectrometry (UPLC-TOF-MS) … then transferred to semi-preparative LC conditions with MS detection … and subsequent characterization by capillary NMR (CapNMR).

12. Hot Off the Press: Natural Products Workflow Herein we present a method of extract evaluation using HPLC profiling with biological evaluation 16 followed by capillary probe NMR spectroscopy/ESMS/UV combined with NMR database (AntiMarin) evaluation, which reduces the crude extract requirement for dereplication to submilligram quantities. 17

13. Full Robotic Automation Automated Push-Button Discrete Sample Loading vs. You pick