3. PLANT DRUG ANALYSIS BY OPTIMUM PERFORMANCE LAMINAR CHROMATOGRAPHY Jacques POTHIER, Laboratory of Pharmacognosy Tours, France North Carolina University july 14 th 2003

4. OPLC 50

5. historique École hongroise Erno TYIYAK Emil MINCOVICS CCM rapide En fait HPLC sur colonne plate

6. Teflon sheet Sorbent bed (silica) Metal cassette External pressure 50 bars Solvent in OPLC principles: the « flat column » concept Micro groove Way out

7. From OPLC 1 to OPLC 3 In the begining OPLC was known as Overpressured layered chromatography –1 chrompress 25 –2 personal oplc –3 to day OPLC is optimal performance laminar chromatography Forget the previous methods –The third generation is dependable, realiable and without surprises.

8. What is OPLC ? Planar and liquid chromatographic techniques Hybrid technique between TLC and HPLC Enabling high performance separation of single sample or of several samples in parallel –Laminar mobile phase –Gradient capabilities –Under pressure excluding vapor phase Planar columns –Disposable, convenient providing laminar flow –Industry selection of phases and particle sizes (5µm, 11µm)

9. OPLC 50

10. From OPLC 1 to OPLC 3 In the begining OPLC was known as Overpressured layered chromatography –1 chrompress 25 –2 personal oplc –3 to day OPLC is optimal performance laminar chromatography Enhanced over previous methods. –The current generation is the latest OPLC.

11. Teflon sheet Sorbent bed (silica) Metal cassette External pressure 50 bars Solvent in OPLC principles: the « flat column » concept Micro groove Way out

12. OPLC/HPLC In OPLC the column is always new. With HPLC...? En oplc toujours possible de vérifier la migration With OPLC, all eluents can be used, acidic and basic. Peut servir pour transposition ccm hplc